DDX4 Antibody

• Catalog number
  N1195-100UG
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  DDX4
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 2-5ug/ml
• Notes
  Provided assay concentrations are suggestions only, antibody titration may be required for optimal results.
• Added buffer
  1X Phosphate Buffered Saline (PBS), pH 7.4
• Intented use
  This DDX4 antibodyis to be used only for research purposes and not for diagnostics..
• Gene ID
  54514
• Purity
  Purified by protein A Affinity Chromatography
• Description
  DEAD box proteins are RNA helicases involved in cellular processes involving the alteration of RNA secondary structure. DDX4, DEAD box polypeptide 4, is the mammalian homolog of the Drosophila VASA gene. DDX4 is expressed solely in ovarian and testicular germ cells and therefore a useful germ cell lineage marker and possible useful biomarker for germ cell cancers.
• Immunogen
  A recombinant protein fragment from the C-terminal region of DDX4/VASA was used as the immunogen for the DDX4 antibody.
• Storage
  Aliquot the DDX4 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  DDX4  
• Gene symbol
  DDX4
• Short name
  Anti-DDX4
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to DDX4
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:18700
• Gene
  DDX4
• Long gene name
  DEAD-box helicase 4
• Synonyms gene name
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Synonyms
  • VASA
• GenBank acession
  • AF262962
• Locus
  5q11.2
• Discovery year
  2002-05-30
• Entrez gene record
  54514
• Pubmed identfication
  • 10920202
  • 11850529
• RefSeq identity
  • NM_024415
• Classification
  • DEAD-box helicases
• VEGA ID
  OTTHUMG00000097044

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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