Protein A Antibody
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Catalog numberbs-0362R
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PricePlease ask
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Size0.1ml
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Long nameProtein A Primary Polyclonal Antibody
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Also known asProtein A PAb
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CategoryPrimary Antibodies
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ConjugationUnconjugated
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Target AntigenProtein A
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SpecificityThis is a highly specific antibody against Protein A.
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Modification sNone
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Modification site sUnmodified antibody
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ClonalityPolyclonal antibody
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Clone numberPolyclonal antibody
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Concentration1ug per 1ul
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Subcellular locationExtracellular
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SourceRecombinant protein A
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Tested ApplicationsWB, IHC-P, IF(IHC-P)
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Recommended dilutionsWB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
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Cross reactivityBacteria
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Cross reactive species detailsS. aureus
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Background of the target antigenProtein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.
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Purification methodThis antibody was purified via Protein A.
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Storage conditionsKeep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
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Synonym namesImmunoglobulin G binding protein A; Staphylococcal protein A; SPA; gG-binding protein A.
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PropertiesIf you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolCENPA, VAPA
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Short nameAnti-Protein A
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TechniqueAntibody, antibodies against human proteins, antibodies for
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HostRabbit (Oryctolagus cuniculus)
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IsotypeImmunoglobulin G (IgG)
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Alternative nameProtein A Polyclonal Antibody
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene namecentromere protein A
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Synonyms gene name
- centromere protein A (17kD)
- centromere protein A, 17kDa
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1988-05-31
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Entrez gene record
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RefSeq identity
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Classification
- Constitutive centromere associated network
- H3 histones
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameVAMP associated protein A
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Synonyms gene name
- VAMP (vesicle-associated membrane protein)-associated protein A (33kD)
- VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
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Synonyms
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Locus
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Discovery year1999-03-19
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Entrez gene record
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Pubmed identfication
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data