Protein A Antibody

• Catalog number
  bs-0362R
• Price
  Please ask
• Size
  0.1ml

• Long name
  Protein A Primary Polyclonal Antibody
• Also known as
  Protein A PAb
• Category
  Primary Antibodies
• Conjugation
  Unconjugated
• Target Antigen
  Protein A
• Specificity
  This is a highly specific antibody against Protein A.
• Modification s
  None
• Modification site s
  Unmodified antibody
• Clonality
  Polyclonal antibody
• Clone number
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Subcellular location
  Extracellular
• Source
  Recombinant protein A
• Tested Applications
  WB, IHC-P, IF(IHC-P)
• Recommended dilutions
  WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
• Cross reactivity
  Bacteria
• Cross reactive species details
  S. aureus
• Background of the target antigen
  Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.
• Synonym names
  Immunoglobulin G binding protein A; Staphylococcal protein A; SPA; gG-binding protein A.
• Properties
  If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Protein  
• Gene symbol
  CENPA, VAPA
• Short name
  Anti-Protein A
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Host
  Rabbit (Oryctolagus cuniculus)
• Isotype
  Immunoglobulin G (IgG)
• Alternative name
  Protein A Polyclonal Antibody
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:1851
• Gene
  CENPA
• Long gene name
  centromere protein A
• Synonyms gene name
  • centromere protein A (17kD)
  • centromere protein A, 17kDa
• Synonyms
  • CENP-A
  • CenH3
• Synonyms name
  • centromere-specific histone
  • histone H3-like centromeric protein A
• GenBank acession
  • U14518
• Locus
  2p23.3
• Discovery year
  1988-05-31
• Entrez gene record
  1058
• RefSeq identity
  • NM_001809
• Classification
  • Constitutive centromere associated network
  • H3 histones
• VEGA ID
  OTTHUMG00000097073

Gene info

• Identity
  HGNC:12648
• Gene
  VAPA
• Long gene name
  VAMP associated protein A
• Synonyms gene name
  • VAMP (vesicle-associated membrane protein)-associated protein A (33kD)
  • VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
• Synonyms
  • hVAP-33
  • VAP-A
• Locus
  18p11.22
• Discovery year
  1999-03-19
• Entrez gene record
  9218
• Pubmed identfication
  • 9920726
  • 9657962
• VEGA ID
  OTTHUMG00000131603

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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