V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5)

Products_type

Antibody
Products_short_name

[V5 Epitope Tag]
Products_name_syn

[Anti -V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5)]
Clonality

Monoclonal
Clone

[3H3115]
Specificity

Recognizes the V5 epitope tag. Detects recombinant proteins tagged with the epitope, some of which include transmembrane and secreted proteins. Usually, a 14aa tag is added to the recombinant proteins, although a smaller epitope of 9aa (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined).
Purity

Affinity Purified Purified by Protein G affinity chromatography.
Form

Supplied as a liquid in PBS, 0.09% sodium azide.
Storage_stability

May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Tested_application

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Radioimmunoassay (RIA)

Gene target

Epitope Tag Pk-tag Paramyxovirus SV5
Short name

V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5)
Technique

Tags
Host

Mouse
Isotype

IgG2a
Label

tag
Alternative name

V5 Epitope detection labelled (Pk-detection labelled, Paramyxovirus, SV5)

Name

Epitope Mapping
Concept

Scope note: Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry.
Tree numbers
- E05.478.274
- E05.601.690.300
Qualifiers

ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data