V5 epitope tag (Pk-tag, paramyxovirus, SV5) (FITC) Antibody

  • Catalog number
    GEN608537
  • Price
    Please ask
  • Size
    100ug
  • Also known as
    V5 epitope tag
  • Other names
    N/A
  • Category
    Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    N/A
  • Gene name synonims
    N/A
  • Other gene names
    N/A
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    IgG2a
  • Clone
    3H3115
  • Host organism
    Mouse (Mus musculus)
  • Species reactivity
    N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined. ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    Affinity Purified by Protein G affinity chromatography.
  • Form Appearance
    Purified IgG conjugated to FITC. Supplied as a liquid, 0.5% BSA, 0.1% sodium azide.
  • Concentration
    N/A
  • Storage and shipping
    Store the antibody at -20 degrees Celsius.
  • Tested applications
    Immunofluorescence (IF)
  • Properties
    If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
  • Conjugation
    Anti-FITC Antibody
  • French translation
    anticorps
  • Gene target
  • Short name
    V5 epitope tag (Pk-tag, paramyxovirus, SV5) (FITC) Antibody
  • Technique
    Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Tags
  • Label
    tag
  • Alternative name
    Anti-V5 epitope tag (Pk-tag, paramyxovirus, SV5) (FITC)
  • Alternative technique
    antibodies, fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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