V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5) (FITC)
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Catalog number
GEN607932
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Price
Please ask
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Size
100ug
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Also known as
V5 Epitope Tag
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Other names
N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
9i131
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Host organism
Mouse (Mus musculus)
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Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein G affinity chromatography.
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Form Appearance
Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide, 1% BSA. Labeled with FITC.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. FITC conjugates are sensitive to light.
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Tested applications
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
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Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Short name
V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5) (FITC)
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Technique
FITC, Fluorescein, Tags
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Label
tag
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Alternative name
Anti-V5 Epitope Tag (Pk-tag, Paramyxovirus, SV5) (FITC)
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Alternative technique
fluorescine
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MeSH Data
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Name
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Concept
Scope note:
A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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