β-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric)
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Catalog number
K733-100
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Price
Please ask
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Size
100 assays
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Description
Convenient & sensitive assay to measure NAG activity
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Summary
• Detection method- Absorbance (400 nm) • Application- Measurement of NAG in biological samples from different mammalian species
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Detection Method
Absorbance (400 nm)
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Applications
• Measurement of NAG in biological samples from different mammalian species
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Sample Type
• Biological fluids such as urine, serum etc. • Tissues such as kidney, liver, brain etc. • Cells such as HeLa, HepG2, CHO etc.
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Features Benefits
• Convenient & sensitive • The assay can detect as low as 50 µU of NAG activity in a variety of samples
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Storage Conditions
-20°C
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Shipping Conditions
gel pack
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Shelf life
12 months
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Background
β-N-Acetylglucosaminidase (NAG, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidney, liver and lungs. NAG can cleave N-acetyl-glucosamine, a monosaccharide derivative of glucose. Its concentration in urine is minimal due to its inability to cross the glomerular basal membrane. Increased concentration of NAG in urine indicates renal tubular cell breakdown. Acute Kidney Injury (AKI) is the sudden loss of kidney functions, causing electrolyte imbalance, and retention of urea and other nitrogenous products. NAG has become one of the most studied and used biomarkers for the detection and diagnosis of AKI. BioVision’s NAG Activity Assay Kit provides a simple and sensitive method for monitoring NAG enzymatic activity. In this assay, NAG uses a synthetic p-nitrophenol derivative (R-pNP) as a NAG substrate and releases pNP which can be measured at absorbance (OD 400 nm). The assay can detect as low as 50 µU of NAG activity in a variety of samples.
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Test
Biovision supplies other types of Assays as 1.
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Properties
Colorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Short name
β-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric)
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Technique
colorimetric, Assay, assays, colorimetry
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Host
Assay
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Alternative name
β-N-Acetylglucosaminidase Activity test reagent (colorimetry)
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Alternative technique
assays, arrays, kits
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Alternative to gene target
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Product images
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