V-ATPase, B, vacuolar H+-ATPase subunit B

  • Catalog number
    AS14 2775
  • Price
    Please ask
  • Size
    50 µg
  • Available ordering format
    Inquire
  • Immunogen
    KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana V-ATPase subunit B, isoform B1: UniProt: Q683E8, TAIR: AT1G76030, isoform B2 UniProt: Q9SZN1, TAIR: AT4G38510, isoform B3: UniProt: Q8W4E2 , TAIR: AT1G20260
  • Raised in
    Rabbit
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified serum in PBS pH 7.4
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
  • Verified applications
    western blot (WB)
  • Connected products
    collection of antibodies to vacuolar proteins AS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPaseAS09 468 | anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa)AS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit DAS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPaseAS09 499 | anti-V-ATPase subunit H |V-type proton ATPase subunit HPlant protein extraction bufferSecondary antibodies
  • Recommended dilutions for use
    1: 1000 with ECL (WB)
  • Molecular weight expected аpparent
    53 | 57 kDa (Vigna radiata)
  • Verified reactivity
    Arabidopsis halleri, Arabidopsis thaliana, Nicotiana tabaccum , Thellungiella salsuginea, Vigna radiata
  • Possible reactivity
    Acetabularia sp. , Arundo donax, Chlamydomonas reinhardtii, Glycine soja, Gossypium mexicanum, Halostachys caspica, Haloxylon ammodendron, Hordeum vulgare, Medicago truncatula, Mesembryantheum crystallinum, Ostreococcus tauri, Oryza sativa, Panax ginseng, Physcomitrella patens, Pinus sylvestris, Populus trichocarpa, Pyrus sp., Ricinus communis, Theobroma cacao, Triticum aestivum, Zea mays, Zostera marina
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
  • References
    to be added when available, antibody released in June 2015
  • Scientific context
    V-ATPase, subunit B is a non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. Alternative names: Vacuolar proton pump subunit B1, vacuolar H+-ATPase subunit B, V-ATPase 57 kDa subunit.
  • Notes
    Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of vacuolar membranes can be found here.
  • Protein number
    Q683E8 , Q9SZN1 , Q8W4E2
  • TAIR number
    AT1G76030,AT4G38510,AT1G20260
  • Gene target
  • Gene symbol
    ATP6V0B, ATP6V1B2
  • Short name
    V-ATPase, B, vacuolar H+-ATPase subunit B
  • Alternative name
    V-ATPase, B, vacuolar H+-ATPase functionnal sequence B
Gene info
  • Identity
  • Gene
  • Long gene name
    ATPase H+ transporting V0 subunit b
  • Synonyms gene
  • Synonyms gene name
    • ATPase, H+ transporting, lysosomal (vacuolar proton pump) 21kD
    • ATPase, H+ transporting, lysosomal 21kDa, V0 subunit c''
    • ATPase, H+ transporting, lysosomal 21kDa, V0 subunit b
  • Synonyms
  • Synonyms name
  • GenBank acession
  • Locus
  • Discovery year
    1997-08-18
  • Entrez gene record
    533
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • V-type ATPase subunits
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    ATPase H+ transporting V1 subunit B2
  • Synonyms gene
  • Synonyms gene name
    • ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide, 56/58kD, isoform 2
    • ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2
    • ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
  • Synonyms
  • Synonyms name
  • GenBank acession
  • Locus
  • Discovery year
    1990-07-03
  • Entrez gene record
    526
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • V-type ATPase subunits
    • ATPase F1/V1 alpha/A and beta/B subunit family
  • VEGA ID
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