Urease, Urease, Biotin conjugated

• Catalog number
  AS09 560
• Price
  Please ask
• Size
  1 ml

• Available ordering format
  Lyophilized in phosphate buffer saline
• Immunogen
  urease isolated and purified from Canavalia ensiformis (common jack-beans)
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Purified IgG
• How to reconstitute
  For reconstitution please add 1 ml of sterile destilled water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), ELISA (ELISA),immunofluorescence (IF), immunohistochemistry (IHC)
• Connected products
  AS09 559 | anti-ureaseAS09 561 | anti-urease (affinity purified)Plant protein extraction buffer
• Recommended dilutions for use
  1: 1000 - 1: 30 000 for techniques listed above
• Molecular weight expected аpparent
  91 kDa
• Verified reactivity
  Canavalia ensiformis (common jack-bean)
• Possible reactivity
  dicots including: Arabidopsis thaliana, Glycine max, Ricinus communis
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
• References
  to be added when available
• Scientific context
  Urease (EC=3.5.1.5) is an enzyme involved in nitrogen metabolism. It is catalyzes the hydrolysis of urea into carbon dioxide and ammonia.
• Notes
  Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated

• Gene target
  Urease   Urease   conjugated  
• Short name
  Urease, Urease, conjugated
• Technique
  antibody Conjugates
• Label
  Biotin
• Alternative name
  Urease, Urease, biotinilated coupled

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