Ubiquitin Antibody, ALEXA FLUOR 555

• Catalog number
  bs-1549R-A555
• Price
  Please ask
• Size
  100ul

• Long name
  Ubiquitin Polyclonal Antibody, ALEXA FLUOR 555 Conjugated
• Also known as
  Anti-Ubiquitin PAb ALEXA FLUOR 555
• Category
  Conjugated Primary Antibodies
• Conjugated with
  ALEXA FLUOR® 555
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  Ubiquitin
• Specificity
  This is a highly specific antibody against Ubiquitin.
• Modification
  Unmodified
• Modification Site
  None
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen range
  5-55/685
• Subcellular location
  Cytoplasm, Nucleus
• Source
  This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Ubiquitin
• Gene ID Number
  7316
• Swiss Prot
  P0CG48
• Tested applications
  IF(IHC-P), IF(ICC)
• Recommended dilutions
  IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat, Pig
• Cross reactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Background of the antigen
  Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
• Purification
  Purified by Protein A.
• Storage conditions
  Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
• Excitation emission
  553nm/568nm
• Synonyms
  HMG2; Polyubiquitin-C; UBC
• Properties
  For facs or microscopy Alexa 1 conjugate. Very high photo stable ALEXA conjugate. If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Conjugation
  Alexa Fluor, ALEXA FLUOR 555
• French translation
  anticorps

• Gene target
  Ubiquitin  
• Gene symbol
  UCHL3, UBE2S, UBE2W
• Short name
  Anti-Ubiquitin
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Immunoglobulin G (IgG)
• Label
  ALEXA FLUOR 555
• Alternative name
  Ubiquitin Polyclonal Antibody, ALEXA FLUOR 555
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:12515
• Gene
  UCHL3
• Long gene name
  ubiquitin C-terminal hydrolase L3
• Synonyms gene name
  • ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
• Synonyms name
  • ubiquitin thiolesterase
• GenBank acession
  • M30496
• Locus
  13q22.2
• Discovery year
  1991-07-15
• Entrez gene record
  7347
• Pubmed identfication
  • 2530630
• RefSeq identity
  • NM_006002
• Classification
  • Ubiquitin C-terminal hydrolases
• VEGA ID
  OTTHUMG00000017090

Gene info

• Identity
  HGNC:17895
• Gene
  UBE2S
• Long gene name
  ubiquitin conjugating enzyme E2 S
• Synonyms gene name
  • ubiquitin-conjugating enzyme E2S
• Synonyms
  • E2-EPF
• Synonyms name
  • ubiquitin carrier protein
  • ubiquitin-conjugating enzyme E2-24 kD
  • ubiquitin-protein ligase
• GenBank acession
  • BC004236
• Locus
  19q13.42
• Discovery year
  2004-01-12
• Entrez gene record
  27338
• Pubmed identfication
  • 1379239
• RefSeq identity
  • NM_014501
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000180811

Gene info

• Identity
  HGNC:25616
• Gene
  UBE2W
• Long gene name
  ubiquitin conjugating enzyme E2 W
• Synonyms gene name
  • ubiquitin-conjugating enzyme E2W (putative)
  • ubiquitin conjugating enzyme E2 W (putative)
• Synonyms
  • FLJ11011
  • UBC-16
• Synonyms name
  • E2 ubiquitin-conjugating enzyme W
  • N-terminal E2 ubiquitin-conjugating enzyme
  • N-terminus-conjugating E2
  • Ubiquitin carrier protein W
  • Ubiquitin-conjugating enzyme 16
  • Ubiquitin-protein ligase W
• GenBank acession
  • AK001873
• Locus
  8q21.11
• Discovery year
  2005-10-06
• Entrez gene record
  55284
• Pubmed identfication
  • 16368532
  • 21229326
  • 26601958
• RefSeq identity
  • NM_001001481
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000164517

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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