UBC9 Antibody (UBE2I)

• Catalog number
  F40817-0.08ML
• Price
  Please ask
• Size
  0.08 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  UBC9 (UBE2I)
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
• Notes
  Titration of the UBC9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This UBC9 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P63279
• Purity
  Purified
• Description
  UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.
• Immunogen
  A portion of amino acids 1-30 from the human protein was used as the immunogen for this UBC9 antibody.
• Storage
  Aliquot the UBC9 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  UBC9   UBE2I  
• Gene symbol
  UBE2I
• Short name
  Anti-UBC9 (UBE2I)
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to UBC9 (UBE2I)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:12485
• Gene
  UBE2I
• Long gene name
  ubiquitin conjugating enzyme E2 I
• Synonyms gene name
  • ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)
  • ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
  • ubiquitin-conjugating enzyme E2I
• Synonyms
  • UBC9
• GenBank acession
  • D45050
• Locus
  16p13.3
• Discovery year
  1995-07-11
• Entrez gene record
  7329
• Pubmed identfication
  • 8565643
• RefSeq identity
  • NM_003345
• Classification
  • Ubiquitin conjugating enzymes E2
• VEGA ID
  OTTHUMG00000186701

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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