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Category
Proteins
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Antibody Subtype
Conjugated Proteins
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Area of research
Proteases, Inhibitors, & Enzymes
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Reactivity
Human
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Residues
161-647 amino acids: MGSSHHHHHH SSGLVPRGSH MYDYDLIIIG GGSGGLAAAK EAAQYGKKVM VLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQA LQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVA LREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLG IPGDKEYCIS SDDLFSLPYC PGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQI EAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYI YAIGDILEDK VELTPVAIQA GRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENI EVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTI GIHPVCAEVF TTLSVTKRSG ASILQAGC
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Conjugate Tag
His tag
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 95% pure
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Concentration
0.5 mg/ml (lot specific)
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Form Buffer
Provided as a liquid in PBS, pH7.4, containing 10% glycerol.
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Storage
Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
SDS-PAGE
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Bioactivity
Specific activity is 0.48-0.6 units/ml, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
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Assay Information
1. Prepare a 0.7ml reaction mixture into a suitable container: The final concentrations are 100mM potassium phosphate, 10mM EDTA, 0.2mM beta-NADPH, 0.05% BSA, 0.014% (w/v) thioredoxin, 5mM DTNB, 5ug, human TRXNRD1. 2. Equilibrate to 25 DEG C and monitor the A412nm until the value is constant using a spectrophotometer. 3. Add 35ul of 100mM DTNB into reaction mixture and mix immediately. 4. Record the increase in A412nm for 2 minutes.
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Additional Information
TXNRD1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Conjugation
histidine
