CHO Host Cell Proteins Elisa kit

• Catalog number
  E4537-100
• Price
  Please ask
• Size
  100 assays

• Description
  A Sandwich ELISA kit for the quantitative measurement of CHO host cell protein in serum, plasma, culture supernatants and other biological fluids.
• Summary
  • Detection method- Absorbance (450 nm) • Species reactivity- Universal • Application- quantitative measurement of CHO HCP in serum, plasma, tissue homogenates and other biological fluids.
• Detection Method
  Absorbance (450 nm)
• Species Reactivity
  Universal
• Applications
  This ELISA kit is used for quantitative measurement of CHO HCP in serum, plasma, tissue homogenates and other biological fluids.
• Sample Type
  Serum, plasma, tissue homogenates and other biological fluids.
• Features Benefits
  Easy, convenient and time-saving method to measure the level of CHO HCP in serum, plasma, tissue homogenates, culture supernatants and other biological fluids. • Detection Range: 0.1 - 100 ng/ml • Sensitivity: < 0.31 ng/ml • Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10% • This assay has high sensitivity and excellent specificity for detection of CHO HCP. No significant cross-reactivity or interference between CHO HCP and analogues was observed.
• Storage Conditions
  4°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  CHO cell line is widely used in the expression of therapeutic protein , which is a cost effective method for production of commercial quantities of a drug substance. One of the goal in designing a downstream process for biopharmaceutical products is to remove any possible contaminants, including host cell proteins. HCPs can still be present at significant concentrations following several purification steps and can even be co-purified and concentrated with the drug substance itself. HCP in the products will result in adverse toxic or immunological reactions which compromise product safety and quality. This it is desirable to reduce the host cell protein contamination to the lowest level. Therefore, during the development of a downstream process, suitable assays must be available to determine the amount of HCP present.. BioVision’s CHO HCP ELISA kit is a sandwich ELISA assay for the quantitative measurement of CHO HCP in Human serum, plasma and cell culture supernatants. The density of color is proportional to the amount of CHO HCP captured from the samples.
• Data Sheet Link
• Additional description
  For cells, cell lines and tissues in culture till half confluency. Most polyclonal antibodies pabs are raised in rabbits as rabbit polyclonal antibodies.
• Properties
  E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
• Test
  ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

• Gene target
  CHO   Proteins  
• Short name
  CHO Proteins Elisa kit
• Technique
  ELISA kit, ELISA, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, mouse, … proteins are supplied in coated 96 well plates to be stored at +4°C. ELISA test kits can be sandwich ELISA. In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies. In single antibody ELISAs the antigen is coated and only a detector antibody is used. Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen. In this case the ratio on your graph will be inverse proportional with the antigen present in your sample. A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion. Genprice Inc. the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800. MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay. PROMT code 3841327 Immunoabsorbent assay tests. ELISA tests are enzyme linked immunoassays to detect human, mouse or other proteins in serum, plasma, urine or biological fluids. MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits. Also Enzyme-linked immunoassay, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330. This is the desigation of other diagnostic analyzers and tests.
• Host
  cho, antigen assay ELISA kit 1 Kit (96 Wells)
• Alternative name
  CHO production species cellular Proteins Enzyme-linked immunosorbent assay reagent
• Alternative technique
  elisas, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  cell

MeSH Data

• Name
  Enzyme-Linked Immunosorbent Assay
• Concept
  Scope note: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
• Tree numbers
  • E05.478.566.350.170
  • E05.478.566.380.360
  • E05.478.583.400.170
  • E05.601.470.350.170
  • E05.601.470.380.360
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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