TurboTEV Protease, Recombinant

• Catalog number
  9205-1
• Price
  Please ask
• Size
  1 mg

• Synonyms
  TEV Protease
• Alternative_names
  Protease
• Description
  A restriction grade protease which cleaves between Gln and Gly
• Recombinant
  Yes
• Source
  E. coli
• Assay
  SDS-PAGE
• Unit Definition
  ≥10,000 units/mg. (One unit cleaves >85% of 3 µg control substrate, Glu-Asn-Leu-Tyr-Phe-Gln-Gly, in 1 hour at 30°C).
• Molecular Weight
  52.0 kDa
• Storage Temp
  -20°C
• Shipping
  Gel Pack
• Shelf Life
  12 months
• Appearance
  Liquid
• Physical form description
  2 mg (20,000 units)/ml in 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM TCEP, 50% glycerol.
• Background Information
  BioVision’s TurboTEV Protease is a cysteine protease that recognizes the cleavage site of Glu-Xaa- Xaa-Y- Xaa-Gln-(Gly/Ser) and cleaves between Gln and Gly; the optimal sequence is Glu-Asn-Leu-Tyr-Phe-Gln-Ser. It contains an enhanced form of a catalytic fragment of the N1a protein of Tobacco etch virus (TEV), TurboTEV. TurboTEV Protease is a restriction grade protease that has robust activity at 4°C with high specificity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. TurboTEV Protease is a 52 kDa protein with both GST and His tags so it can be removed by either Ni-chelating or glutathione (GSH) resin.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  TurboTEV   Protease  
• Short name
  TurboTEV Protease, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  TurboTEV Protease, Rec.
• Alternative technique
  rec

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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