Turbo3C (HRV3C) Protease, Recombinant
-
Catalog number
9206-1
-
Price
Please ask
-
Size
1 mg
-
-
Synonyms
HRV3C Protease
-
Alternative_names
Protease
-
Description
A restriction grade protease which cleaves at the PreScission Site
-
Recombinant
Yes
-
Source
E. coli
-
Assay
SDS-PAGE
-
Unit Definition
> 1 unit/µg. One unit of Turbo3C (HRV3C) Protease cleaves >95% of 100 µg of target protein at 4°C for 16 hours
-
Molecular Weight
47.0 kDa
-
Storage Temp
-20°C
-
Shipping
Gel Pack
-
Shelf Life
12 months
-
Appearance
Liquid
-
Physical form description
2 mg/ml in 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM TCEP, and 50% glycerol
-
Background Information
BioVision’s Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly (independent of Pro). The recombinant form of the HRV3C protease is a restriction grade protease that has robust activity at 4°C with high specific activity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. This HRV3C Protease is a 47 kDa protein with both GST and His tags so it can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Short name
Turbo3C (HRV3C) Protease, Recombinant
-
Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Alternative name
Turbo3C (HRV3C) Protease, Rec.
-
Alternative technique
rec
-
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products