Caspase 8 antibody

Category

Primary Antibody
Antibody Subtype

Polyclonal Antibodies
Area of research

Cell Cycle & Cell Death
Type of Immunogen

Caspase 8 antibodies were raised in Rabbit using synthetic peptide surrounding the cleavage site of human Caspase-8 ..
Raised in

Rabbit
Specificity

The affinity purified antibody mainly detects the 26 kDa fully cleaved fragment and the 35-43 kDa intermediate of Caspase-8 in apoptotic samples.
Cross Reactivity

Human
Method of Purification

Caspase 8 antibody was purified by affinity purification.
Concentration

500 ug/ml
Form Buffer

Provided in PBS buffer, pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Storage

Store at -20 deg C. For long-term storage, aliquot and freeze at -70 deg C. Avoid repeated freeze/thaw cycles.
Shipping conditions

Blue Ice
Tested for

WB
Usage Recommendations

WB: 0.5-4 ug/ml
Assay Information

Caspase 8 Blocking Peptide, catalog no. 33R-10456, is also available for use as a blocking control in assays to test for specificity of this Caspase 8 antibody
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description

Human and some mouse caspases are active in apoptosis and cell death and even in necrosis and inflammation. CASP Gene and orthologous enzymes have been identifies successfully in the signal transduction cascade and pathways.
French translation

anticorps

Identity

HGNC:1509
Gene

CASP8
Long gene name

caspase 8
Synonyms gene name
- caspase 8, apoptosis-related cysteine protease
- caspase 8, apoptosis-related cysteine peptidase
Synonyms
- MCH5
- MACH
- FLICE
- Casp-8
GenBank acession
- U60520
Locus

2q33.1
Discovery year

1996-11-11
Entrez gene record

841
Pubmed identfication
- 8681376
- 8681377
RefSeq identity
- NM_001228
Classification
- Caspases
- Death effector domain containing
- Death inducing signaling complex
- Ripoptosome
VEGA ID

OTTHUMG00000132821
Locus Specific Databases
- CASP8base: Mutation registry for Caspase 8 deficiency
- LRG_34

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data