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Available ordering format
Lyophilized in PBS pH 7.4
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Immunogen
KLH-conjugated peptide derived from available tubulin beta chain sequences (located at a surface loop) including all Arabidopsis thaliana tubulin beta-2/beta-3 chain P29512, beta-4 chain P24636(At5g44340), beta-5 chain P29513(At1g20010), beta-6 chain P29514(At5g12250), beta-7 chain P29515(At2g29550), beta-8 chain P29516(At5g23860), beta-9 chain P29517(At4g20890).
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified serum
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How to reconstitute
For reconstitution add 100 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
immunofluorescence (IF), western blot (WB)
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Connected products
AS10 680 | tubulin alpha chainPlant and algal protein extraction bufferSecondary antibodies
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Recommended dilutions for use
1: 1000 (IF), 1:500 (WB)
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Molecular weight expected аpparent
49 | 49 kDa (Arabidopsis thaliana)
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Verified reactivity
Arabidopsis thaliana (including suspension cells), Chlamydomonas reinhardii, Medicago sativa
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Possible reactivity
dicots including: Brassica napus, Glycine max, Pisum sativum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Vitis vinifera, monocots including: Hordeum vulgare, Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens, microalgae:Micromonoas pusilla, Ostreococcus lucimarinus, S. cerevisiae
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No reactivity
no confirmed exceptions from predicted reactivity known in the moment
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Supplementary information
to be added when available
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References
Heinnickel et al. (2016). Tetratricopeptide repeat protein protects photosystem I from oxidative disruption during assembly. Proc Natl Acad Sci U S A. 2016 Mar 8;113(10):2774-9. doi: 10.1073/pnas.1524040113
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Scientific context
Tubulin beta (TUB) together with alpha tubulin is making up microtubules.
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Notes
Please, note that tubulin beta is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to allow detection on a western blot, analyzed material must contain enough meristematic cells with dividing activity.Signal in a western blot application in Chlamydomonas reinhardtii is obtained with a load of 100 µg/well and a dilution of 1: 500.
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Protein number
P29512 , P24636 , P29513 , P29514 , P29515 , P29516 , P29517
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TAIR number
AT5G62690
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