Tubulin, alpha chain

• Catalog number
  AS10 680
• Price
  Please ask
• Size
  100 µl

• Available ordering format
  Lyophilized
• Immunogen
  KLH-conjugated peptide derived from available tubulin alpha chain sequences including Arabidopsis thaliana tubulin alpha-1-chain P11139(At1g64740), alpha-2/alpha-4 chain B9DGT7(At1g50010), alpha-5 chain B9DHQ0(At5g19780), alpha-6-chain P29511(At4g14960)
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 100 µl of sterile water
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), immunofluorescence (IF)
• Connected products
  AS10 681 | tubulin beta chainPlant and algal protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 1000 with standard ECL (WB), 1: 500 (IF)
• Molecular weight expected аpparent
  49 | 52 kDa (Arabidopsis thaliana)
• Verified reactivity
  Arabidopsis thaliana, Hordeum vulgare, Zea mays
• Possible reactivity
  dicots including: Brassica napus, Glycine max, Pisum sativum, Solanum tuberosum, Sorghum bicolor, Ricinus communis, Vitis vinifera, monocots including: Triticum aestivum, Oryza sativa, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens, algae: Chlorella vulgaris, Euglena gracilis, Micromonoas pusilla, Ostreococcus lucimarinus
• No reactivity
  no confirmed exceptions from predicted reactivity known in the moment
• Supplementary information
  Peptide used to elict this antibody is not present in tubulin beta.
• References
  Juszczak et al. (2012). Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol. Plant.
• Scientific context
  Tubulin alpha (TUA) together with beta tubulin is making up microtubules.
• Notes
  Please, note that tubulin alpha is only detected in actively dividing meristematic cells (see immunolocalization image below). Therefore to unable detection on a western blot, analyzed material must contain enough cells with dividing activity.
• Protein number
  P11139 , P29510 , P20363 , P29511
• TAIR number
  AT1G64740
• Hyperlink
• Description
  The Tubulin, alpha chain is a α- or alpha protein sometimes glycoprotein present in blood.

• Gene target
  Tubulin   alpha   chain  
• Short name
  Tubulin, alpha chain
• Alternative name
  Tubulin, a epitope

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