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Target antigen
Troponin T
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Clonality
Monoclonal antibody
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Clone
JLT-12
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Raised in
mice
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Type of the antibody
IgG1 monoclonal antibody
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Product form
freeze-dried
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Reacts with species
Human, rat, chicken
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Analyses
WB,IHC-P,IHC-F
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Immunogen
Troponin T from rabbit skeletal muscle.
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Product configuration
Mouse ascites fluid, 1.2% Na acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
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Purification
Ascites
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Solubilization
The powdered antibody should be dissolved in 1ml of PBS buffer to achieve final concentration of 100ug/ml.
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Storage condtions
Keep the Troponin T Antibody ( JLT12)at minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.
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Tips
The Troponin T Antibody ( JLT12) did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.
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Background
The troponin complex is located on the thin filament of striated muscle and is composed of 3 component polypeptides: troponin 1, troponin T and troponin C. Three troponin T genes have been described on the basis of molecular cloning in humans and other vertebrates. These are expressed in a tissue-specific manner and encode the troponin T isoforms expressed in slow skeletal muscle(TNNT1), cardiac muscle(TNNT2),and fast skeletal muscle(TNNT3). TNNT1, TNNT2, TNNT3 are located on 19q13.4, 1q32, 11p15.5 respectively.
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Related articles
1. Samson, F.; de Jong, P. J.; Trask, B. J.; Koza-Taylor, P.; Speer, M. C.; Potter, T.; Roses, A. D.; Gilbert, J. R. : Assignment of the human slow skeletal troponin T gene to 19q13.4 using somatic cell hybrids and fluorescence in situ hybridization analysis. Genomics 13: 1374-1375, 1992. 2. Durand, J.-B.; Bachinski, L. L.; Bieling, L. C.; Czernuszewicz, G. Z.; Abchee, A. B.; Yu, Q. T.; Tapscott, T.; Hill, R.; Ifegwu, J.; Marian, A. J.; Brugada, R.; Daiger, S.; Gregoritch, J. M.; Anderson, J. L.; Quinones, M.; Towbin, J. A.; Roberts, R. : Localization of a gene responsible for familial dilated cardiomyopathy to chromosome 1q32. Circulation 92: 3387-3389, 1995. 3. Mao, C.; Baumgartner, A. P.; Jha, P. K.; Huang, T. H.-M.; Sarkar, S. : Assignment of the human fast skeletal troponin T gene (TNNT3) to chromosome 11p15.5: evidence for the presence of 11pter in a monochromosome 9 somatic cell hybrid in NIGMS mapping panel 2. Genomics 31: 385-388, 1996.
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Gene Name
TNNT1; TNNT2; TNNT3
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Protein Name
Troponin T, slow skeletal muscle; Troponin T, cardiac muscle; Troponin T, fast skeletal muscle
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Gene Full Name
troponin T type 1(skeletal, slow); troponin T type 2(cardiac); troponin T type 3(skeletal, fast)
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Synonyms
TNT|TNTF
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Uniprot ID
Human(P13805), Mouse(O88346), Rat(Q7TNB2), Human(P45379), Mouse(P50752), Rat(P50753), Human(P45378), Mouse(Q9QZ47), Rat(P09739)
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Entrez GeneID
Human(7138), Mouse(21955), Rat(171409), Human(7139), Mouse(21956), Rat(24837), Human(7140), Mouse(21957), Rat(24838)
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Properties
If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
