-
About recombinant protein
TRAIL Receptor-1/DR4 and TRAIL Receptor-2/DR5 belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic "death domain", which can activate the cell's apoptotic machinery. These receptors are activated by binding to either membrane anchored or soluble TRAIL/Apo2L. Recombinant human soluble TRAIL Receptor-2/DR5 is a 14.9 kDa protein (133 amino acid residues) consisting of the TNFR homologous, cysteine rich portion of the extracellular domain.
-
Other name of the recombinant
DR5, CD262, KILLER, TRICK2, TRICKB, ZTNFR9, TRAILR2, TRICK2A, TRICK2B, TRAIL-R2, KILLER/DR5, DR5, UNQ160/PRO186, Tumor necrosis factor receptor superfamily member 10B, Death receptor 5, TRAIL receptor 2
-
Recombinant in
E. coli
-
SEQUENCE
MESALITQQD LAPQQRVAPQ QKRSSPSEGL CPPGHHISED GRDCISCKYG QDYSTHWNDL LFCLRCTRCD SGEVELSPCT TTRNTVCQCE EGTFREEDSP EMCRKCRTGC PRGMVKVGDC TPWSDIECVH KES
-
Activity
Biological activity was determined by its inhibitory effect of IL - 8 production in human PBMC by human sTRAIL/Apo2L. 50% inhibition (100 ng/mL of sTRAIL/Apo2L) was reached using a concentration of 1000 ng/mL of sTRAIL Receptor - 2.
-
Keep at
The lyophilized Soluble TRAIL Receptor-2 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Soluble TRAIL Receptor-2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Soluble TRAIL Receptor-2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
-
Rec PROTEIN GI
224494019
-
Accession nr
NP_003833.4
-
Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
-
About
This product is for research use only.
-
Protein s origin
Human
-
Vial
Lyophilized
-
NCBI ID
8795
-
Gene name
tumor necrosis factor receptor superfamily, member 10b
-
Source
Homo sapiens
-
Uniprot
O14763
-
-
-
Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants