Total Protein - Murine Embryonic Stem Cell Line D3
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Catalog number
CBA-305
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Price
Please ask
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Size
500 μg
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We send this reagant on
Dry Ice
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The temperatureerature for storage is
-80ºC; avoid freeze/thaw
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SDS or safty data sheet
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Type of reagent
Stem Cell Research
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Related techniques
Stem Cell Research, ES Cell Total Protein
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Cellular use
Isolated from mouse ES-D3 cell line
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Protocol information
Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer
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Conjugation and form
host specific
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Species compatible to
human cells and cell lines and other cells
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Use of this test kit or product
Molecular biology
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Antigen
the sequence of the protein
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Buffer
The concentration in mg/ml in a 10mM of buffered solution at pH 7 to 7,59
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Molecular weight
kiloDalton
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This reagent is purified
0.98
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Description
For cells, cell lines and tissues in culture till half confluency.
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Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
MeSH Data
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Name
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Concept
Scope note:
Methods or procedures used to obtain samples of URINE.
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Tree numbers
- E01.370.225.998.762
- E05.200.998.762
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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