Total Bile Acids (TBA) Assay Kit (Colorimetric)

• Catalog number
  K209-100
• Price
  Please ask
• Size
  100 assays

• Description
  A simple, sensitive & easy colorimetric assay to quantitate total bile acids.
• Summary
  • Detection method- Absorbance (405 nm). • Species reactivity- Mammalian • Applications- Estimation of Bile Acids in various biological samples.
• Detection Method
  Absorbance (405 nm).
• Species Reactivity
  Mammalian
• Applications
  Estimation of Bile Acids in various biological samples.
• Sample Type
  • Biological fluids such as serum, plasma, bile, urine, saliva, etc.
• Features Benefits
  • Simple, fast and convenient assay • Can detect as low as 1 µM Bile acids in a variety of samples.
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Bile Acids (BA) make 67% of the total composition of Bile. They are 24-carbon steroids generated during cholesterol metabolism. They form conjugates with either glycine or taurine to form bile salts. Five of the BAs account for more than 99% of the total population found in biofluids. The average composition in healthy individuals includes conjugates of cholic, chenodeoxycholic, deoxycholic and lithocholic acids. Bile acids are critical due to their ability to solubilize lipids by forming micelles with cholesterol, and fatty acids. Their synthesis is not only critical for the removal of cholesterol from the body abut they are also needed for proper uptake of dietary lipids into the small intestine. The measurement of circulatory Total Bile Acids (TBA) therefore provides information about hepatic functions and liver diseases such as jaundice, and hepatocellular injury. TBA estimation can detect liver damage during early stages and permits patients to get treatment before hepatic damages become irreversible. In addition, Bile Acids participate as signaling molecules interacting with G-protein coupled receptors (GPCR), TGR5, and nuclear receptor farnesoid X receptor (FXR). BioVision’s Total Bile Acid Assay Kit provides a simple, sensitive, and high-throughput adaptable approach to detect physiological concentration of total bile acids in a variety of biological fluids. The principle of the assay is based on an enzymatic cycling method in the presence of NADH and a chromophore. The reduction of the chromophore produces a stable colorimetric product the absorbance of which can be followed kinetically at 405 nm. This absorbance is directly proportional to the amount of TBA in the sample. Our assay is very specific and sensitive. Other metabolites found in biofluids do not interfere with the assay. The assay can detect as little as 1 µM of Bile Acids in a variety of samples.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Bile   Acids   TBA   Kit  
• Short name
  Bile Acids (TBA) Assay Kit (Colorimetric)
• Technique
  colorimetric, Assay, assays, colorimetry
• Host
  Assay
• Alternative name
  Total Bile Acids (TBA) test reagent (colorimetry)
• Alternative technique
  assays, arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  total

Product images

Similar products