Total Antioxidant Capacity (TAC) Colorimetric Assay Kit
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Catalog numberK274-100
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PricePlease ask
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Size100 assays
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Summary• Detection method- Absorbance (570 nm) • Species reactivity- Mammalian • Applications- Assay measuring either the combination of both small molecule and protein antioxidants or small molecules antioxidant alone in the presence of our proprietary Protein Mask.
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Sample TypeCell and tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
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Features Benefits• Simple procedure; takes less than 2 hours • Fast and convenient
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Storage Conditions4°C
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Shipping Conditionsgel pack
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Shelf life12 months
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BackgroundAntioxidants play an important role in preventing the formation of and scavenging of free radicals and other potentially toxic oxidizing species. There are three categories of antioxidant species: enzyme systems (GSH reductase, catalase, peroxidase, etc.), small molecules (ascorbate, uric acid, GSH, vitamin E, etc.) and proteins (albumin, transferrin, etc.). Different antioxidants vary in their reducing power. Trolox is used to standardize antioxidants, with all other antioxidants being measured in Trolox equivalents. Measurement of the combined nonenzymatic antioxidant capacity of biological fluids and other samples provides an indication of the overall capability to counteract reactive oxygen species (ROS), resist oxidative damage and combat oxidative stress-related diseases. In some cases, the antioxidant contribution of proteins is desired whereas in other cases only the contribution of the small molecule antioxidants is needed. BioVision developed the TAC Assay Kit, which can measure either the combination of both small molecule antioxidants and proteins or small molecules alone in the presence of our proprietary Protein Mask. Cu²ᶧ ion is converted to Cu+ by both small molecule and protein. The Protein Mask prevents Cu²ᶧ reduction by protein, enabling the analysis of only the small molecule antioxidants. The reduced Cu+ ion is chelated with a colorimetric probe giving a broad absorbance peak around 570 nm, proportional to the total antioxidant capacity.
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TestBiovision supplies other types of Assays as 1.
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PropertiesColorimetric assays or detection use UV absorption or enzymatic color reaction.
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Gene target
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Gene symbolTRV-TAC1-2, TRV-TAC1-1, TRV-TAC2-1, TRV-TAC3-1, TRV-TAC4-1, CXCL11, NMTRV-TAC1-1
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Short nameAntioxidant Capacity (TAC) Colorimetric Assay Kit
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Techniquecolorimetric, Assay, assays, colorimetry
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HostAssay
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Alternative nameTotal Antioxidant Capacity (TAC) colorimetry test reagent
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Alternative techniqueassays, arrays, kits
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Alternative to gene targetv-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
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Tissuetotal
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Gene info
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Identity
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Gene
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Long gene nametRNA-Val (anticodon TAC) 1-2
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Synonyms gene
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Synonyms gene name
- transfer RNA valine 19 (anticodon UAC)
- transfer RNA-Val (TAC) 1-2
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Val (anticodon TAC) 1-1
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Synonyms gene
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Synonyms gene name
- transfer RNA valine 11 (anticodon UAC)
- transfer RNA-Val (TAC) 1-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-27
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Val (anticodon TAC) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA valine 23 (anticodon UAC)
- transfer RNA-Val (TAC) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Val (anticodon TAC) 3-1
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Synonyms gene
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Synonyms gene name
- transfer RNA valine 26 (anticodon UAC)
- transfer RNA-Val (TAC) 3-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Val (anticodon TAC) 4-1
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Synonyms gene
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Synonyms gene name
- transfer RNA valine 31 (anticodon UAC)
- transfer RNA-Val (TAC) 4-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nameC-X-C motif chemokine ligand 11
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Synonyms gene
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Synonyms gene name
- small inducible cytokine subfamily B (Cys-X-Cys), member 11
- chemokine (C-X-C motif) ligand 11
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Synonyms
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GenBank acession
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Locus
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Discovery year1998-06-23
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Entrez gene record
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Pubmed identfication
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Classification
- Chemokine ligands
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namenuclear-encoded mitochondrial tRNA-Val (TAC) 1-1
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Synonyms gene
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Synonyms gene name
- transfer RNA valine 37 (anticodon UAC) pseudogene
- nuclear-encoded mitochondrial transfer RNA-Val (TAC) 1-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Nuclear-encoded mitochondrial transfer RNAs
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
