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Available ordering format
Lyophilized
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Immunogen
Recombinant Toc159 (C-terminally His-tagged, including the central GTPase domain but lacking the N-terminal acidic domain as well as the C-terminal membrane associating domain) from Pisum sativum Toc159 protein sequence (Q9LKR1)
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Raised in
Guinea pig
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Serum
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How to reconstitute
For reconstitution add 50 µl of sterile water
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Storage condition
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Verified applications
western blot (WB), immunolocalization (IL)
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Connected products
AS06 150 | anti-Toc75 (monocots and dicots)AS08 292 | anti-Toc75 (Pisum sativum)
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Recommended dilutions for use
1 : 10 000 with alkaline phosphatase (WB), 1: 500 (CM)
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Molecular weight expected аpparent
150 kDa (with protease inhibitors), 86 and 53 kDa (following proteolysis) and approx. 29 kDa degradation product (cross reaction with GTPase domain)
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Verified reactivity
Pisum sativum
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Possible reactivity
dicots, Oryza sativa
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No reactivity
Arabidopsis thaliana
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Supplementary information
Toc159 is extremely sensitive to proteolytic processing in vivo therefore use of protease inhibitors is of crucial importance.
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References
to be added when available
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Scientific context
Toc159 is located in the outer chloroplast membrane and part of of the Toc/TOC (translocon of the outer envelope membrane of chloroplasts) complex. It mediates the GTP-dependent initial stages of preprotein import into chloroplasts. In Arabidopsis thaliana, Toc159 is encoded by a small gene family: atTOC159, atTOC132, atTOC120, and atTOC90. Phylogenetic analysis suggested that at least two distinct Toc159 subtypes, characterized by atToc159 and atToc132/atToc120, exist in plants.
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Notes
Antibodies have been used in fluorescence and confocal microscopy and total internal reflectance confocal microscopy.
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Protein number
Q9LKR1
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TAIR number
Refer to NCBI
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Description
Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae.
