Synonyms TNF, Tumor necrosis factor- alpha
Alternates names Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
Taglines A potent cytokine exerting cytotoxic effects on a wide range of cells
NCBI Gene ID #. 21926
NCBI Gene Symbol TNFA
Gene Source Mouse
Accession # P06804.2
Source E. coli.
Activity (Specifications/test method) N/A
Biological activity The ED₅₀ as determined by the cytolysis of murine L929 cells in the presence of actinomycin D is <0.1 ng/ml, corresponding to a specific activity of> 1 x 10⁷ units/mg.
Molecular Weight 17.5 kDa
Storage Temp. -20°C
Shipping gel pack
Shelf Life 1 year
Appearance Lyophilized powder
Physical form description Steriled and lyophilized from 5 mM Tris, pH 8.0.
Reconstitution Instructions Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans
Description The TNF-alpha, murine recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
Additional source Recombinants or rec. proteins
Gene Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
Short name TNF-alpha, murine recombinant
Alternative name tumor necrosis factor-a, murine recombinant
Alternative technique rec
Alternative to gene target tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943