TNF-alpha, human recombinant
-
Catalog number1050-1000
-
PricePlease ask
-
Size1 mg
-
-
SynonymsTNF, Tumor necrosis factor- alpha
-
Alternative_namesTumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
-
DescriptionA potent cytokine exerting cytotoxic effects on a wide range of cells
-
RecombinantYes
-
SourceE. coli.
-
Purity by SDS PAGE≥98%
-
AssaySDS-PAGE
-
Purity≥98%
-
Biological activityED50 < 0.05 ng/ml as determined by the cytolysis of murine L929 cells in the presence of actinomycin D.
-
Molecular Weight17.4 kDa
-
Storage Temp-20°C
-
Shippinggel pack
-
Shelf Life12 months
-
AppearanceLyophilized powder
-
Physical form descriptionLyophilized in 50 mM Tris, 150 mM NaCl pH 8.5
-
Reconstitution InstructionsReconstitute in H₂O to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
-
Background InformationTumor Necrosis Factor-α(TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF-α is a 17.4 kDa protein containing 157 amino acid residues.
-
HandlingCentrifuge the vial prior to opening.
-
UsageFor Research Use Only! Not to be used in humans
-
Additional descriptionThe TNF-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
-
PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Additional sourceRecombinants or rec. proteins
-
Grouprecombinants
-
GeneTumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
-
Gene target
-
Gene symbolTNF, TNFRSF1A, TNFRSF1B, LTBR
-
Short nameTNF-alpha, recombinant
-
TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
SpeciesHuman, Humans
-
Alternative nametumor necrosis factor-a, H. sapiens Rec.
-
Alternative techniquerec
-
Alternative to gene targettumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943
-
Gene info
-
Identity
-
Gene
-
Long gene nametumor necrosis factor
-
Synonyms gene
-
Synonyms gene name
- tumor necrosis factor (TNF superfamily, member 2)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1986-01-01
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Tumor necrosis factor superfamily
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nameTNF receptor superfamily member 1A
-
Synonyms gene
-
Synonyms gene name
- tumor necrosis factor receptor superfamily, member 1A
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1991-01-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- Tumor necrosis factor receptor superfamily
- CD molecules
-
VEGA ID
-
Locus Specific Databases
Gene info
-
Identity
-
Gene
-
Long gene nameTNF receptor superfamily member 1B
-
Synonyms gene
-
Synonyms gene name
- tumor necrosis factor receptor superfamily, member 1B
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year1991-01-15
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- CD molecules
- MicroRNA protein coding host genes
- Tumor necrosis factor receptor superfamily
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namelymphotoxin beta receptor
-
Synonyms gene
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1996-03-12
-
Entrez gene record
-
Pubmed identfication
-
Classification
- Tumor necrosis factor receptor superfamily
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data