TNF-alpha, human recombinant

  • Catalog number
    1050-1000
  • Price
    Please ask
  • Size
    1 mg
  • Synonyms
    TNF, Tumor necrosis factor- alpha
  • Alternative_names
    Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
  • Description
    A potent cytokine exerting cytotoxic effects on a wide range of cells
  • Recombinant
    Yes
  • Source
    E. coli.
  • Purity by SDS PAGE
    ≥98%
  • Assay
    SDS-PAGE
  • Purity
    ≥98%
  • Biological activity
    ED50 < 0.05 ng/ml as determined by the cytolysis of murine L929 cells in the presence of actinomycin D.
  • Molecular Weight
    17.4 kDa
  • Storage Temp
    -20°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Appearance
    Lyophilized powder
  • Physical form description
    Lyophilized in 50 mM Tris, 150 mM NaCl pH 8.5
  • Reconstitution Instructions
    Reconstitute in H₂O to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
  • Background Information
    Tumor Necrosis Factor-α(TNF-α) is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF-α is a 17.4 kDa protein containing 157 amino acid residues.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Additional description
    The TNF-alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene
    Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.
  • Gene target
  • Gene symbol
    TNF, TNFRSF1A, TNFRSF1B, LTBR
  • Short name
    TNF-alpha, recombinant
  • Technique
    Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Species
    Human, Humans
  • Alternative name
    tumor necrosis factor-a, H. sapiens Rec.
  • Alternative technique
    rec
  • Alternative to gene target
    tumor necrosis factor, DIF and TNF-alpha and TNFA and TNFSF2, TNF and IDBG-300259 and ENSG00000232810 and 7124, transcription regulatory region DNA binding, Extracellular, Tnf and IDBG-177358 and ENSMUSG00000024401 and 21926, TNF and IDBG-634161 and ENSBTAG00000025471 and 280943
Gene info
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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