Storage Conditions -20°C
Tag Line Sensitive, Colorimetric Assay
Shipping Conditions gel pack
Shelf life 12 months
Species Reactivity human
Applications Quantitative protein detection, establishing normal range, validation of antibody array results
Sample Type • Serum & plasma (human TNF-alpha concentration is pretty low in normal serum & plasma, it may not be detected in this assay) • Cell culture supernatants • Urine
Synonyms TNF, Tumor necrosis factor- alpha
Alternates names Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2
Taglines A potent cytokine exerting cytotoxic effects on a wide range of cells
NCBI Gene ID #. 7124
NCBI Gene Symbol TNFA
Gene Source Human
Accession # P01375
Source E. coli.
Endotoxin Level N/A
Activity (Specifications/test method) N/A
Biological activity ED50 <0.05 ng/ml as determined by the cytolysis of murine L929 cells in the presence of actinomycin D.
Molecular Weight 17.4 kDa
Storage Temp. -20°C
Shipping gel pack
Shelf Life 1 year
Appearance Lyophilized powder
Physical form description Lyophilized in 50 mM Tris, 150 mM NaCl pH 8.5
Reconstitution Instructions Reconstitute in H₂O to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.
Handling Centrifuge the vial prior to opening.
Usage For Research Use Only! Not to be used in humans