TMRE Mitochondrial Membrane Potential Assay Kit (Fluorometric)

• Catalog number
  K238-200
• Price
  Please ask
• Size
  200 assays

• Description
  Simple assay to measure mitochondrial membrane potential
• Summary
  • Detection method- Fluorescence (Ex/Em = 535/587 nm), FACS [FL-3 (Red) channel], Fluorescent microscopy [red filter (Ex/Em = 549/575 nm)] • Applications- Labeling of active mitochondria in cells - Screening of compounds which may compromise mitochondrial membrane potential
• Detection Method
  Fluorescence (Ex/Em = 535/587 nm), FACS [FL-3 (Red) channel], Fluorescent microscopy [red filter (Ex/Em = 549/575 nm)]
• Applications
  • Labeling of active mitochondria in cells • Screening of compounds which may compromise mitochondrial membrane potential
• Sample Type
  • Adherent and suspension cells
• Features Benefits
  • Convenient method • Based on cell-permeable, positively-charged, red-orange dye TMRE, that readily accumulates in the active mitochondria • Includes Protonophores FCCP to validate the assay
• Storage Conditions
  -20°C
• Shipping Conditions
  gel pack
• Shelf life
  12 months
• Background
  Mitochondria are the powerhouse of the cells and generate energy in the form of ATP. Mitochondria utilize oxidizable substrates to produce a membrane potential in the form of a proton gradient across the mitochondrial inner membrane. Mitochondrial membrane potential is tightly interlinked to many mitochondrial processes, including ATP synthesis, ROS, calcium sequestration and import of proteins into the mitochondrion. Therefore, pharmacological changes in mitochondrial membrane potential are associated with several pathological parameters. BioVision’s TMRE Mitochondrial Membrane Potential Assay Kit uses TMRE (tetramethylrhodamine, ethyl ester) to label active mitochondria. TMRE is a cell-permeable, positively-charged, red-orange dye that readily accumulates in active mitochondria. Protonophores like FCCP and CCCP induces dissipation of mitochondrial membrane thus results in compromised staining.
• Data Sheet Link
• Test
  Biovision supplies other types of Assays as 1.
• Additional description
  Associated membrane protein types are lipopolysaccharide selective barriers. Biological membranes include cell membranes, outer coverings of cells or organelles that allow passage of certain proteins and nuclear membranes, which cover a cell nucleus; and tissue membranes, such as mucosae and serosae. 

• Gene target
  TMRE   Mitochondrial   Membrane   Potential   Kit   Fluorometric  
• Short name
  TMRE Mitochondrial Potential Assay Kit (Fluorometric)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  TMRE Mitochondrial Membrane Potential test reagent (fluorescence)
• Alternative technique
  arrays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832
• Tissue
  mitochondrial

MeSH Data

• Name
  Radioallergosorbent Test
• Concept
  Scope note: An in vitro allergen radioimmunoassay in which allergens are coupled to an immunosorbent. The coupled allergens bind the IgE in the sera of patients which in turn binds radioisotope-labeled anti-IMMUNOGLOBULIN E antibodies.
• Tree numbers
  • E01.370.225.812.735.830
  • E05.200.812.735.830
  • E05.478.566.380.810
  • E05.478.566.639.810
  • E05.478.594.760.830
  • E05.601.470.380.810
  • E05.601.470.639.810
• Qualifiers
  ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data

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