TLR4 Antibody: FITC

• Catalog number
  SPC-200D-FITC
• Price
  Please ask
• Size
  100 µg

• Alernative name
  Rabbit Anti-Human TLR4 Polyclonal
• Target
  TLR4
• Conjugate
  FITC
• Research Area s
  Immunology , Innate Immunity , Macrophage , Immunology , Immunoglobulins , Receptors , Cardiovascular System , Atherosclerosis , Vascular Inflammation , Inflammatory Mediators , Immunology , Adaptive Immunity , Regulatory T Cells , Immunology , Innate Immunity , TLR Signaling
• Category
  Antibodies
• Product Type
  Polyclonal
• Immunogen
  Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4
• Immunogen Species
  Human
• Gene ID
  7099
• Swiss Prot
  O00206
• Applications
  WB , IHC , ICC/IF , FCM , FACS
• Host Species
  Rabbit
• Species Reactivity Full Name
  Human , Mouse
• Storage Buffer
  PBS, 50% glycerol, 0.09% sodium azide
• Concentration
  1 mg/ml
• Specificity
  Detects ~75-80kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).
• Storage Temperature
  -20°C
• Shipping Temperature
  Blue Ice or 4°C
• Properties
  If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  TLR4  
• Gene symbol
  TLR4
• Short name
  TLR4 Antibody: FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Label
  FITC
• Alternative name
  toll-like receptor 4 (antibody to-): fluorecein
• Alternative technique
  antibodies, fluorescine
• Alternative to gene target
  toll-like receptor 4, ARMD10 and CD284 and TLR-4 and TOLL, TLR4 and IDBG-82738 and ENSG00000136869 and 7099, protein binding, Cell surfaces, Tlr4 and IDBG-156729 and ENSMUSG00000039005 and 21898, TLR4 and IDBG-638758 and ENSBTAG00000006240 and 281536

Gene info

• Identity
  HGNC:11850
• Gene
  TLR4
• Long gene name
  toll like receptor 4
• Synonyms gene name
  • toll-like receptor 4
• Synonyms
  • hToll
  • CD284
  • TLR-4
  • ARMD10
• GenBank acession
  • U88880
• Locus
  9q33.1
• Discovery year
  1998-06-25
• Entrez gene record
  7099
• Pubmed identfication
  • 9435236
  • 9237759
• RefSeq identity
  • NM_138554
• Classification
  • Toll like receptors
  • CD molecules
• VEGA ID
  OTTHUMG00000021046
• Locus Specific Databases
  • LRG_320

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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