TIP1;1,TIP1;2, tonoplast intrinistic protein1-1, 1-2 (gamma)
-
Catalog numberAS09 492
-
PricePlease ask
-
Size100 µl
-
-
Available ordering formatInquire
-
ImmunogenKLH-conjugated synthetic peptide conserved in Raphanus sativus TIP1;1 and TIP1;2 (protein accesion number available in Suga et al. 2001)
-
Raised inRabbit
-
ClonalityPolyclonal
-
ClonePolyclonal
-
PurificationSerum
-
How to reconstituteSee included datasheet or contact our support service
-
Storage conditionstore at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
-
Verified applicationsELISA (ELISA), western blot (WB)
-
Connected productscollection of antibodies to tonoplast proteins AS09 482 | anti-AtTIP1;1 | gamma TIPAS09 493 | anti-TIP1;1, TIP1;2 | tonoplast intrinistic protein 1-1, 1-2 (gamma)AS09 494 | anti-TIP2;1 (delta) | Delta-VM23AS09 510 | anti-TIP2;1 | tonoplast intrinistic protein 2-1AS09 511 | anti-TIP2;2 | tonoplast intrinistic protein 2-2 (C-terminal)Plant protein extraction bufferSecondary antibodies
-
Recommended dilutions for use1: 8000 (ELISA), 1: 1000 with standard ECL (WB)
-
Molecular weight expected аpparent25.8| 23 kDa
-
Verified reactivityArabidopsis thaliana, Raphanus sativus
-
Possible reactivitydicots including: Brassica napus
-
No reactivityno confirmed exceptions from predicted reactivity known in the moment
-
Supplementary informationProtein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Manufactured by Operon Biotechnologies.
-
ReferencesSuga et al. (2001). Specificity of the accumulation of mRNAs and proteins of the plasma membrane and tonoplast aquaporins in radish organs. Planta 212:294-304.
-
Scientific contextTIPs belong to MIP/aquaporin protein family. Alternative names: gamma-tonoplast intrinsic protein, gamma-TIP, aquaporin TIP, tonoplast intrinsic protein, root-specific RB7, gamma-tonoplast intrinsic protein 2, gamma-TIP2, salt stress-induced tonoplast intrinsic protein
-
Notes0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.Protocol for isolation of vacuolar membranes can be found here.
-
Protein numberRefer to NCBI
-
TAIR numberRefer to NCBI
-
Gene target
-
Gene symbolIGHVIII-2-1, MIR1-2, TRUND-NNN9-1, SLC2A4RG, TRH-GTG2-1, MIR511, TRN-ATT1-2, TRS-AGA2-1, TRG-CCC2-1, TRC-GCA2-2
-
Short nameTIP1 1 TIP1 2, tonoplast intrinistic protein1-1, 1-2 (gamma)
-
Alternative nameTIP1;1,TIP1;2, tonoplast intrinistic protein1-1, 1-2 (g)
-
Gene info
-
Identity
-
Gene
-
Long gene nameimmunoglobulin heavy variable (III)-2-1 (pseudogene)
-
Synonyms gene name
- immunoglobulin heavy variable (III)-2-1
- immunoglobulin heavy variable (III)-2-1 pseudogene
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2000-04-17
-
Entrez gene record
-
RefSeq identity
-
Classification
- Immunoglobulin heavy locus at 14q32.33
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 1-2
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR1/206 family
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-undetermined (NNN) 9-1
-
Synonyms gene name
- transfer RNA-undetermined (NNN) 9-1
-
GenBank acession
-
Locus
-
Discovery year2014-06-20
-
Entrez gene record
-
Pubmed identfication
Gene info
-
Identity
-
Gene
-
Long gene nameSLC2A4 regulator
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year2001-06-21
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-His (GTG) 2-1
-
Synonyms gene
-
Synonyms gene name
- transfer RNA histidine 11 (anticodon GUG)
- transfer RNA-His (GTG) 2-1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2008-08-29
-
Entrez gene record
-
Classification
- Low confidence cytoplasmic transfer RNAs
Gene info
-
Identity
-
Gene
-
Long gene namemicroRNA 511
-
Synonyms gene
-
Synonyms gene name
- microRNA 511-2
- microRNA 511-1
-
Synonyms
-
Locus
-
Discovery year2005-07-15
-
Entrez gene record
-
RefSeq identity
-
Classification
- MicroRNA MIR506 family
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-Asn (ATT) 1-2
-
Synonyms gene name
- transfer RNA-Asn (ATT) 1-2
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2014-07-07
-
Entrez gene record
-
Classification
- Low confidence cytoplasmic transfer RNAs
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-Ser (anticodon AGA) 2-1
-
Synonyms gene
-
Synonyms gene name
- transfer RNA serine 25 (anticodon AGA)
- transfer RNA-Ser (AGA) 2-2
- transfer RNA-Ser (AGA) 2-1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2008-08-29
-
Entrez gene record
-
Classification
- Cytoplasmic transfer RNAs
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-Gly (anticodon CCC) 2-1
-
Synonyms gene
-
Synonyms gene name
- transfer RNA glycine 22 (anticodon CCC)
- transfer RNA-Gly (CCC) 2-2
- transfer RNA-Gly (CCC) 2-1
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2008-08-29
-
Entrez gene record
-
Classification
- Cytoplasmic transfer RNAs
Gene info
-
Identity
-
Gene
-
Long gene nametRNA-Cys (anticodon GCA) 2-2
-
Synonyms gene
-
Synonyms gene name
- transfer RNA cysteine 5 (anticodon GCA)
- transfer RNA-Cys (GCA) 2-1
- transfer RNA-Cys (GCA) 2-2
-
Synonyms
-
GenBank acession
-
Locus
-
Discovery year2008-08-29
-
Entrez gene record
-
Classification
- Cytoplasmic transfer RNAs