TIMP-1 Recombinant Protein
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Catalog number
40-141
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Price
Please ask
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Size
0.002 mg, 0.01 mg
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About recombinant protein
TIMP1 is an extracellular inhibitor of MMPs (see above) including MMP1, 2, 3, 7, 8, 9, 10, 11, 12, 13, and 16. It belongs to the I35 (TIMP) family of irreversible protease inhibitors that function as key modulators of extracellular matrix degradation during tissue development and remodeling. TIMP1 can also act through an MMP-independent mechanism to promote erythropoiesis by stimulating proliferation and differentiation of erythroid progenitors. Recombinant human TIMP-1 is a 20.6 kDa protein containing 184 amino acid residues.
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Other name of the recombinant
EPA, EPO, HCI, CLGI, TIMP, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, EPA
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Recombinant in
E. coli
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SEQUENCE
CTCVPPHPQT AFCNSDLVIR AKFVGTPEVN QTTLYQRYEI KMTKMYKGFQ ALGDAADIRF VYTPAMESVC GYFHRSHNRS EEFLIAGKLQ DGLLHITTCS FVAPWNSLSL AQRRGFTKTY TVGCEECTVF PCLSIPCKLQ SGTHCLWTDQ LLQGSEKGFQ SRHLACLPRE PGLCTWQSLR SQIA
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Activity
TIMP1 activity was measured by its ability to inhibit human MMP - 1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP - 1 concentration of approximately 0.5 μg/mL, when using an MMP - 1 concentration of 1.6 μg/mL.
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Keep at
The lyophilized TIMP-1 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted TIMP-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing TIMP-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
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Rec PROTEIN GI
4507509
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Accession nr
NP_003245.1
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Pureity
Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
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About
This product is for research use only.
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Protein s origin
Human
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Vial
Lyophilized
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NCBI ID
7076
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Gene name
TIMP metallopeptidase inhibitor 1
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Source
Homo sapiens
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Uniprot
P01033
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Additional source
Recombinants or rec. proteins
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Group
recombinants
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Gene target
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Gene symbol
TIMP1
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Short name
TIMP-1 Recombinant Protein
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Technique
Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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Alternative name
TIMP-1 Rec. Protein
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Alternative technique
rec
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Gene info
MeSH Data
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Name
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Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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