Flag-Tag Antibody

  • Catalog number
    T0003
  • Price
    Please ask
  • Size
    100µL
  • Type
    primary antibody
  • Shipping
    Ice bag
  • Antigen
    Flag-Tag
  • Reactivity
    All
  • Clonality
    Monoclonal
  • Application
    WB,IHC,IF/ICC,IP,ELISA
  • Application_advice
    WB: 1:3000~1:10000, IF/ICC: 1:200,IP 1:200
  • Conjugate
    Unconjugated
  • Immunogen
    A synthetic peptide DYKDDDDK coupled to KLH.
  • Purification
    Affinity-chromatography.
  • Format
    Liquid
  • Concentration
    1mg/ml
  • Fragment
    Fab fragment
  • Storage_Buffer
    Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
  • Properties
    If you buy Antibodies supplied by Affinity Biosciences they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv.  These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
  • Conjugation
    Flag
  • French translation
    anticorps
  • Gene target
    Tag  
  • Gene symbol
    TRL-TAG2-1, TRL-TAG3-1, TRL-TAG4-1
  • Short name
    -Tag Antibody
  • Technique
    Antibody, antibodies against human proteins, antibodies for, Tags
  • Host
    Mouse
  • Isotype
    IgG1
  • Label
    Flag
  • Alternative name
    Flag-detection labelled (antibody to-)
  • Alternative technique
    antibodies
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Leu (anticodon TAG) 2-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA leucine 15 (anticodon UAG)
    • transfer RNA-Leu (TAG) 2-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Leu (anticodon TAG) 3-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA leucine 7 (anticodon UAG)
    • transfer RNA-Leu (TAG) 3-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • Classification
    • Cytoplasmic transfer RNAs
Gene info
  • Identity
  • Gene
  • Long gene name
    tRNA-Leu (anticodon TAG) 4-1
  • Synonyms gene
  • Synonyms gene name
    • transfer RNA leucine 40 (anticodon UAG) pseudogene
    • transfer RNA-Leu (TAG) 4-1
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2008-08-29
  • Entrez gene record
  • RefSeq identity
  • Classification
    • Cytoplasmic transfer RNA pseudogenes
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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