Thioredoxin 1 (Trx) Fluorometric Substrate, Di- FITC oxidized glutathione (FGSSGF)

• Catalog number
  9699-200
• Price
  Please ask
• Size
  200 assays

• Description
  Ready-to-use fluorogenic substrate for Trx. Trx activity can be quantified by fluorescent detection of oxidized Glutathione after reduction of the disulfide bond at 490/525 nm, using a fluorometer or multi-well fluorescence plate reader.
• Peptide sequence
  FITC-Glutathione-Glutathione-FITC
• Salt Form
  No
• Molecular Weight
  1391.36
• Purity
  >99% by HPLC
• Solubilities
  Resuspend 835 µg in 6 ml of 50 mM sodium phosphate buffer at pH 7.4.
• Handling
  Store at 4°C, protected from light.
• Tag Line
  Thioredoxin 1 (Trx) Fluorometric substrate
• Storage Condition
  +4°C
• Shipping Condition
  gel pack
• Shelf Life
  12 months
• SMILES
  N/A
• InChi
  N/A
• InChi Key
  N/A
• Data Sheet Link
• MSDS Available
  Yes
• Properties
  This Biovision Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Biovision FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Thioredoxin   Trx   Fluorometric   Substrate   oxidized   glutathione   FGSSGF  
• Short name
  Thioredoxin 1 (Trx) Fluorometric Substrate, Di- FITC oxidized glutathione (FGSSGF)
• Technique
  FITC, Fluorescein
• Label
  FITC
• Alternative name
  Thioredoxin 1 (Trx) fluorescence Substrate, Di- fluorecein oxidized GSH (FGSSGF)
• Alternative technique
  fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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