TAK1(Thr187) Antibody, FITC Conjugated

• Catalog number
  bs-3438R-FITC
• Price
  Please ask
• Size
  0.1ml

• Long name
  TAK1(Thr187) Polyclonal Antibody, FITC Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  FITC
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  TAK1 Thr187
• Specificity
  This is a highly specific antibody against TAK1 Thr187.
• Modification
  Phosphorylation
• Modification site
  Thr187
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  180-190/606
• Subcellular location
  Cytoplasm
• Source
  KLH conjugated synthetic phosphopeptide derived from human TAK1 around the phosphorylation site of Thr187
• Gene ID number
  6885
• Swiss Prot
  O43318
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  494nm/518nm
• Synonyms
  TAK1; MEKK7; TGF1a; Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1; TGF-beta-activated kinase 1; MAP3K7
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
• Additional conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  TAK1   Thr187   Conjugated  
• Gene symbol
  MAP3K7, NR2C2
• Short name
  Anti-TAK1 Thr187
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein
• Isotype
  Immunoglobulin G (IgG)
• Label
  FITC
• Alternative name
  Anti-TAK1 Thr187 PAb FITC
• Alternative technique
  antibodies, fluorescine

Gene info

• Identity
  HGNC:6859
• Gene
  MAP3K7
• Long gene name
  mitogen-activated protein kinase kinase kinase 7
• Synonyms gene
  • TAK1
• Synonyms
  • MEKK7
• Synonyms name
  • TGF-beta activated kinase 1
• GenBank acession
  • AB009356
• Locus
  6q15
• Discovery year
  1998-05-22
• Entrez gene record
  6885
• Pubmed identfication
  • 9466656
• RefSeq identity
  • NM_145331
• Classification
  • Mitogen-activated protein kinase kinase kinases
• VEGA ID
  OTTHUMG00000015217
• Locus Specific Databases
  • The Globin Gene Server

Gene info

• Identity
  HGNC:7972
• Gene
  NR2C2
• Long gene name
  nuclear receptor subfamily 2 group C member 2
• Synonyms gene
  • TR4
• Synonyms gene name
  • nuclear receptor subfamily 2, group C, member 2
• Synonyms
  • TAK1
  • TR2R1
  • hTAK1
• GenBank acession
  • L27586
• Locus
  3p25.1
• Discovery year
  1998-05-15
• Entrez gene record
  7182
• Pubmed identfication
  • 8661150
  • 8016112
• RefSeq identity
  • NM_003298
• Classification
  • Nuclear receptor subfamily 2 group C
• VEGA ID
  OTTHUMG00000129839

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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