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Available ordering format
Lyophilized
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Immunogen
T7 tag sequence MASMTGGQQMG.
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinty purified
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How to reconstitute
See included datasheet or contact our support service
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Storage condition
Store at 2-8°C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
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Verified applications
Western blot (WB)
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Connected products
AS15 2976 | T7, ALP conjugated (rabbit antibody)AS15 2983 | T7, DyLight® 488 conjugated (rabbit antibody)AS15 2984 | T7, DyLight® 550 conjugated (rabbit antibody)AS15 2978 | T7, DyLight® 650 conjugated (rabbit antibody)AS15 2982 | T7, SureLight® R-Phycoerythrin conjugated (rabbit antibody)other tag antibodies
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Recommended dilutions for use
to be determined by end user
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
to be added when available, antibody released in August 2015
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Scientific context
Rabbit anti-T7 is a primary antibody which binds to T7 and is directly conjugated to Biotin.
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Notes
Antibody is provided in: 100 mM Sodium Phosphate pH 7.4, 100 mM Sodium Chloride, 50 mM Sucrose, 0.1% BSA and 2 mM Sodium Azide
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
