Sulfo-NHS-Biotin

• Catalog number
  2322-50
• Price
  Please ask
• Size
  50 mg

• Alternative_name
  Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt; Sulfosuccinimidobiotin; Sulfo NHS Biotin;Sulfosuccinimidyl biotin; N-hydroxysulfosuccinimidyl biotin; N-hydroxysulfosuccinimido biotin
• Description
  KEY FEATURES: • Sulfo-NHS-Biotin is amine-reactive and has a spacer arm length of 13.5 Å. • Water-soluble; can be used under physiologic conditions • The amide bond formed is permanent and the alkyl spacer arm cannot be cleaved APPLICATIONS: • Sulfo-NHS-Biotin is ideal for biotinylating antibody or protein that can then be used for protein detection or immobilization • Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent
• CAS Number
  119616-38-5
• Structure Available
  Yes
• Salt Form
  No
• Molecular Formula
  C₁₄H₁₈O₈N₃S₂Na
• Molecular Weight
  443.43
• Cell Permeable
  No
• Purity
  ≥85% by NMR
• Solubilities
  H₂O (~ 20 mg/ml)
• Handling
  Protect from moisture
• Tag Line
  A water-soluble biotinylating reagent
• Storage Condition
  -20 ̊C
• Shipping Condition
  Gel Pack
• Shelf Life
  36 months
• MDL Number
  MFCD00078535
• PubChem CID
  16219034
• PubChem CID Link
• SMILES
  C1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)O.[Na]
• InChi
  InChI=1S/C14H19N3O8S2.Na/c18-10-5-9(27(22,23)24)13(20)17(10)25-11(19)4-2-1-3-8-12-7(6-26-8)15-14(21)16-12;/h7-9,12H,1-6H2,(H2,15,16,21)(H,22,23,24);
• InChi Key
  WEECOOCIRDDPES-UHFFFAOYSA-N
• Data Sheet Link
• MSDS Available
  Yes
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated

• Gene target
  Sulfo-NHS  
• Gene symbol
  NHS, NHS-AS1
• Short name
  Sulfo-NHS-
• Label
  Biotin
• Alternative name
  Sulfo-NHS-biotinilated

Gene info

• Identity
  HGNC:7820
• Gene
  NHS
• Long gene name
  NHS actin remodeling regulator
• Synonyms gene name
  • Nance-Horan syndrome (congenital cataracts and dental anomalies)
• Locus
  Xp22.2-p22.13
• Discovery year
  1989-06-30
• Entrez gene record
  4810
• Pubmed identfication
  • 20332100
• RefSeq identity
  • NM_198270
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000022799
• Locus Specific Databases
  • Global Variome shared LOVD

Gene info

• Identity
  HGNC:40403
• Gene
  NHS-AS1
• Long gene name
  NHS antisense RNA 1
• Synonyms gene name
  • NHS antisense RNA 1 (non-protein coding)
• Locus
  Xp22.13
• Discovery year
  2011-07-29
• Classification
  • Antisense RNAs
• VEGA ID
  OTTHUMG00000021203

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