StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

• Catalog number
  CBA-325
• Price
  Please ask
• Size
  96 reactions

• Type of the product
  Set of reagents
• Product description
  Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the Set of reagents; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.
• Shipping conditions
  The product StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)can be shipped at ambient temperature without any risk for the quality and activity of the reagents.
• Storage conditions
  Our specialists from Gentaur/Genrpice recommend to keep the product at four degrees Celsius to ensure the retention of the quality of the reagent until the exiration date.
• Tips
  This product is manufactured for research purposes only and it is not intended to be used in clinical diagnostic procedures.
• Test
  Cellular biology labs supplies other types of Assays as 1. Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
• Description
  For cells, cell lines and tissues in culture till half confluency. Colonies can be formed by stimulating factors or recombinant GM-CSF and CSFs activity expressed in Units compared to a standard.

• Gene target
  StemTAG   Stem   Colony   Formation   Recovery   Compatible  
• Short name
  StemTAG Stem Colony Formation Assay ( Recovery Compatible)
• Technique
  Assay, assays
• Host
  Assay
• Alternative name
  StemTAG progenitor cellular Colony Formation test (cellular Recovery Compatible)
• Alternative technique
  arrays
• Tissue
  cell, stem

MeSH Data

• Name
  Colony-Forming Units Assay
• Concept
  Scope note: A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
• Tree numbers
  • E01.370.225.500.383
  • E05.200.500.383
  • E05.242.383
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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