Splicing factor 1 Antibody

• Catalog number
  R30963
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Splicing factor 1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, IHC-F, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Splicing factor 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Splicing factor 1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q15637
• Purity
  Antigen affinity
• Description
  Splicing factor 1, also known a sSF1, Zinc finger protein 162 (ZNF162), ZFM162, Transcription factor ZFM1, and Mammalian branch point-binding protein (BBP or mBBP), is a protein that in humans is encoded by the SF1 gene. It ispredicted 623-amino acid protein. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary. All of the isoforms contain a KH domain, a sequence motif present in proteins playinig a major role in regulating cellular RNA metabolism.
• Immunogen
  An amino acid sequence from the N-terminus of human Splicing factor 1 (DFPSKKRKRSRWNQDTMEQK) was used as the immunogen for this Splicing factor 1 antibody (100% homologous in human, mouse and rat).
• Storage
  After reconstitution, the Splicing factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• Additional description
  Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
• French translation
  anticorps

• Gene target
  Splicing   factor  
• Gene symbol
  SRSF1, SF3B1, SF3A1
• Short name
  Anti-Splicing factor 1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Splicing factor 1
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10780
• Gene
  SRSF1
• Long gene name
  serine and arginine rich splicing factor 1
• Synonyms gene
  • SFRS1
• Synonyms gene name
  • splicing factor, arginine/serine-rich 1
  • serine/arginine-rich splicing factor 1
• Synonyms
  • ASF
  • SF2
  • SRp30a
  • SF2p33
  • MGC5228
• Synonyms name
  • splicing factor 2
  • pre-mRNA-splicing factor SF2, P33 subunit
  • alternate splicing factor
  • SR splicing factor 1
• Locus
  17q22
• Discovery year
  1993-09-17
• Entrez gene record
  6426
• Pubmed identfication
  • 8530103
  • 20516191
• RefSeq identity
  • NM_006924
• Classification
  • Serine and arginine rich splicing factors
  • RNA binding motif containing
• VEGA ID
  OTTHUMG00000178781

Gene info

• Identity
  HGNC:10768
• Gene
  SF3B1
• Long gene name
  splicing factor 3b subunit 1
• Synonyms gene name
  • splicing factor 3b, subunit 1, 155kD
  • splicing factor 3b, subunit 1, 155kDa
• Synonyms
  • SAP155
  • SF3b155
  • PRPF10
  • Prp10
  • Hsh155
• GenBank acession
  • AF054284
• Locus
  2q33.1
• Discovery year
  2000-02-29
• Entrez gene record
  23451
• Pubmed identfication
  • 9585501
• Classification
  • SF3b complex
  • B-WICH chromatin-remodelling complex subunits
  • Armadillo like helical domain containing
• VEGA ID
  OTTHUMG00000154447
• Locus Specific Databases
  • LRG_624

Gene info

• Identity
  HGNC:10765
• Gene
  SF3A1
• Long gene name
  splicing factor 3a subunit 1
• Synonyms gene name
  • splicing factor 3a, subunit 1, 120kD
  • splicing factor 3a, subunit 1, 120kDa
• Synonyms
  • SF3a120
  • SAP114
  • PRPF21
  • Prp21
• GenBank acession
  • X85237
• Locus
  22q12.2
• Discovery year
  2000-02-29
• Entrez gene record
  10291
• Pubmed identfication
  • 7489498
• RefSeq identity
  • NM_005877
• Classification
  • SF3a complex
  • U2 small nuclear ribonucleoprotein
• VEGA ID
  OTTHUMG00000151005
• Locus Specific Databases
  • LRG_622

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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