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Category
Proteins
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Antibody Subtype
Blocking Peptides
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Area of research
Signal Transduction
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Type of protein
Synthetic
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Form Buffer
Provided in phosphate buffered saline (PBS), pH 7.2, containing 0.1% BSA and 0.02% thimerosal.
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Storage
Store at -20 deg C for short term storage. Aliquot and store at -70 deg c for long term storage, avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
WB
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Assay Information
The peptide is used for blocking the binding of Smad4 antibody (catalog no. 20R-1588). In optimal conditions, it blocks the binding completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30 minutes at 37 deg C.
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Test
You can block the antibody by the specific target amino acid sequence of peptide.
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Properties
blocking peptide
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Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
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Gene target
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Gene symbol
SMAD4
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Short name
Smad4 Blocking Peptide
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Technique
blocking peptide, Blocking, peptide, blocking peptides, fitzgerald made this blocking amino acid sequence or peptide to block the gene target in a volume of 1. For western blot it is often requested to block your antibody and to see the band of the analyzed protein disappear. This is called a negative control by blocking the WB antibody. peptides
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Alternative name
A synthetic peptide for use as a blocking control in assays to test for specificity of Smad4 antibody, catalog no. 20R-1588
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Alternative technique
control, peptides
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Alternative to gene target
SMAD family member 4, DPC4 and JIP and MADH4 and MYHRS, SMAD4 and IDBG-3485 and ENSG00000141646 and 4089, transforming growth factor beta receptor, nuclei, Smad4 and IDBG-157503 and ENSMUSG00000024515 and 17128, SMAD4 and IDBG-644730 and ENSBTAG00000006919 and 540248
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