Slit2 Recombinant Protein

• Catalog number
  40-494
• Price
  Please ask
• Size
  0.005 mg, 0.025 mg

• About recombinant protein
  Slit2 is a member of the Slit family that signals through the Roundabout (Robo) receptor as a repellent for axon guidance and neuronal migration, and can also act as a chemoattractant to vascular endothelial cells and a chemotaxis inhibitor for leukocytes. Slit2 is expressed primarily in the fetal lung, kidney, and adult spinal cord, and to a lesser extent in adult adrenal gland, thyroid and trachea. Slit2 is initially synthesized as a 1499 amino acid precursor, which is subsequently cleaved into N-terminal and C-terminal fragments, designated as Slit2-N and Slit2-C respectively. The neurodevelopment related activities, as measured by the ability to repel olfactory bulb axons and to induce branching in dorsal root ganglia axons, are contained only in the N-terminal fragment. Recombinant human Slit2-N is a 1088 amino acid glycoprotein corresponding to the N-terminal portion of the full length Slit2 precursor. Due to glycosylation Slit2-N migrates at an apparent molecular weight of approximately 120.0-140.0 kDa by SDS-PAGE analysis under reducing conditions.
• Other name of the recombinant
  SLIL3, Slit-2, SLIL3, Slit homolog 2 protein
• Recombinant in
  HEK293 cells
• SEQUENCE
  ACPAQCSCSG STVDCHGLAL RSVPRNIPRN TERLDLNGNN ITRITKTDFA GLRHLRVLQL MENKISTIER GAFQDLKELE RLRLNRNHLQ LFPELLFLGT AKLYRLDLSE NQIQAIPRKA FRGAVDIKNL QLDYNQISCI EDGAFRALRD LEVLTLNNNN ITRLSVASFN HMPKLRTFRL HSNNLYCDCH LAWLSDWLRQ RPRVGLYTQC MGPSHLRGHN VAEVQKREFV CSGHQSFMAP SCSVLHCPAA CTCSNNIVDC RGKGLTEIPT NLPETITEIR LEQNTIKVIP PGAFSPYKKL RRIDLSNNQI SELAPDAFQG LRSLNSLVLY GNKITELPKS LFEGLFSLQL LLLNANKINC LRVDAFQDLH NLNLLSLYDN KLQTIAKGTF SPLRAIQTMH LAQNPFICDC HLKWLADYLH TNPIETSGAR CTSPRRLANK RIGQIKSKKF RCSAKEQYFI PGTEDYRSKL SGDCFADLAC PEKCRCEGTT VDCSNQKLNK IPEHIPQYTA ELRLNNNEFT VLEATGIFKK LPQLRKINFS NNKITDIEEG AFEGASGVNE ILLTSNRLEN VQHKMFKGLE SLKTLMLRSN RITCVGNDSF IGLSSVRLLS LYDNQITTVA PGAFDTLHSL STLNLLANPF NCNCYLAWLG EWLRKKRIVT GNPRCQKPYF LKEIPIQDVA IQDFTCDDGN DDNSCSPLSR CPTECTCLDT VVRCSNKGLK VLPKGIPRDV TELYLDGNQF TLVPKELSNY KHLTLIDLSN NRISTLSNQS FSNMTQLLTL ILSYNRLRCI PPRTFDGLKS LRLLSLHGND ISVVPEGAFN DLSALSHLAI GANPLYCDCN MQWLSDWVKS EYKEPGIARC AGPGEMADKL LLTTPSKKFT CQGPVDVNIL AKCNPCLSNP CKNDGTCNSD PVDFYRCTCP YGFKGQDCDV PIHACISNPC KHGGTCHLKE GEEDGFWCIC ADGFEGENCE VNVDDCEDND CENNSTCVDG INNYTCLCPP EYTGELCEEK LDFCAQDLNP CQHDSKCILT PKGFKCDCTP GYVGEHCDID FDDCQDNKCK NGAHCTDAVN GYTCICPEGY SGLFCEFSPP MV
• Keep at
  The lyophilized Slit2 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Slit2 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Slit2 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
• Rec PROTEIN GI
  574281382
• Accession nr
  NP_001276065.1
• Pureity
  Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
• About
  This product is for research use only.
• Protein s origin
  Human
• Vial
  Lyophilized
• NCBI ID
  9353
• Gene name
  slit homolog 2 (Drosophila)
• Source
  Homo sapiens
• Uniprot
  O94813
• Pictures of recombinant
• Rec pdf
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Slit2   Protein  
• Gene symbol
  SLIT2
• Short name
  Slit2 Recombinant Protein
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. proscience advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Alternative name
  slit homolog 2 (Drosophila) Rec. Protein
• Alternative technique
  rec
• Alternative to gene target
  slit homolog 2 (Drosophila), SLIL3 and Slit-2, SLIT2 and IDBG-11255 and ENSG00000145147 and 9353, Roundabout binding, Extracellular, Slit2 and IDBG-162862 and ENSMUSG00000031558 and 20563, SLIT2 and IDBG-641256 and ENSBTAG00000005108 and 534164

Gene info

• Identity
  HGNC:41383
• Gene
  SLIT2-IT1
• Long gene name
  SLIT2 intronic transcript 1
• Synonyms gene name
  • SLIT2 intronic transcript 1 (non-protein coding)
• Locus
  4p15.31
• Discovery year
  2011-08-23
• Entrez gene record
  100505893
• Classification
  • Intronic transcripts
• VEGA ID
  OTTHUMG00000160239

Gene info

• Identity
  HGNC:11086
• Gene
  SLIT2
• Long gene name
  slit guidance ligand 2
• Synonyms gene
  • SLIL3
• Synonyms gene name
  • slit (Drosophila) homolog 2
  • slit homolog 2 (Drosophila)
• Synonyms
  • Slit-2
• GenBank acession
  • AF055585
• Locus
  4p15.31
• Discovery year
  1999-06-11
• Entrez gene record
  9353
• Pubmed identfication
  • 9813312
  • 18269211
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000128551

Gene info

• Identity
  HGNC:11087
• Gene
  SLIT3
• Long gene name
  slit guidance ligand 3
• Synonyms gene
  • SLIL2
• Synonyms gene name
  • slit (Drosophila) homolog 3
  • slit homolog 3 (Drosophila)
• Synonyms
  • slit2
  • MEGF5
  • SLIT1
  • Slit-3
• GenBank acession
  • AB011538
• Locus
  5q34-q35.1
• Discovery year
  1998-03-25
• Entrez gene record
  6586
• Pubmed identfication
  • 9693030
  • 9813312
• RefSeq identity
  • NM_003062
• Classification
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000130409

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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