GPI, human recombinant

• Catalog number
  7822-100
• Price
  Please ask
• Size
  100 ug

• Synonyms
  Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36.
• Alternative_names
  Glucose-6-phosphate isomerase, GPI, AMF, GNPI, NLK, PGI, PHI, SA36.
• Description
  A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate
• Recombinant
  Yes
• Source
  E. coli.
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Endotoxin Level
  < 1.0 EU per 1 ug of protein (determined by LAL method)
• Biological activity
  Specific activity is > 90 units/mg, and one unit will convert 1.0 µmole D-fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 25ºC.
• Results
  Specific activity is > 90 units/mg
• Unit Definition
  One unit will convert 1.0 µmole D-fructose 6-phosphate to D-glucose 6-phosphate per minute at pH 7.4 at 25ºC.
• Molecular Weight
  65.3 kDa (578 aa, 1-558 aa + His Tag)
• Storage Temp
  -20°C
• Shipping
  gel pack
• Shelf Life
  12 months
• Concentration
  1 mg/ml
• Appearance
  Liquid
• Physical form description
  1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol and 1 mM DTT
• Background Information
  Glucose-6-phosphate isomerase, also known as GPI, belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Recombinant human GPI, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Amino acid sequence
  MGSSHHHHHH SSGLVPRGSH MAALTRDPQF KLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK FCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT LEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  GPI  
• Gene symbol
  GPI, PIGH, PIGM, GPIHBP1, VNN2, GNPDA1
• Short name
  GPI, recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  glucose-6-phosphate isomerase, H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  glucose-6-phosphate isomerase, AMF and GNPI and NLK and PGI and PHI and SA-36 and SA36, GPI and IDBG-42902 and ENSG00000105220 and 2821, monosaccharide binding, nuclei, GPI and IDBG-635386 and ENSBTAG00000006396 and 280808

Gene info

• Identity
  HGNC:4458
• Gene
  GPI
• Long gene name
  glucose-6-phosphate isomerase
• Synonyms gene name
  • glucose phosphate isomerase
• Synonyms
  • AMF
  • NLK
• GenBank acession
  • M61214
• Locus
  19q13.11
• Discovery year
  2001-06-22
• Entrez gene record
  2821
• Pubmed identfication
  • 2387591
  • 8575767
• VEGA ID
  OTTHUMG00000182087
• Locus Specific Databases
  • LRG_1178

Gene info

• Identity
  HGNC:8964
• Gene
  PIGH
• Long gene name
  phosphatidylinositol glycan anchor biosynthesis class H
• Synonyms gene name
  • phosphatidylinositol glycan, class H
• Synonyms
  • GPI-H
• Synonyms name
  • phosphatidylinositol N-acetylglucosaminyltransferase subunit
• Locus
  14q24.1
• Discovery year
  1994-09-06
• Entrez gene record
  5283
• Pubmed identfication
  • 8204896
• RefSeq identity
  • NM_004569
• Classification
  • Phosphatidylinositol glycan anchor biosynthesis
• VEGA ID
  OTTHUMG00000171806

Gene info

• Identity
  HGNC:18858
• Gene
  PIGM
• Long gene name
  phosphatidylinositol glycan anchor biosynthesis class M
• Synonyms gene name
  • phosphatidylinositol glycan, class M
• Synonyms
  • GPI-MT-I
• Synonyms name
  • GPI mannosyltransferase 1
  • DPM:GlcN-(acyl-)PI mannosyltransferase
  • dol-P-Man dependent GPI mannosyltransferase
• GenBank acession
  • AB028127
• Locus
  1q23.2
• Discovery year
  2002-07-05
• Entrez gene record
  93183
• Pubmed identfication
  • 11226175
• RefSeq identity
  • NM_145167
• Classification
  • Dolichyl D-mannosyl phosphate dependent mannosyltransferases
  • Phosphatidylinositol glycan anchor biosynthesis
• VEGA ID
  OTTHUMG00000024081

Gene info

• Identity
  HGNC:24945
• Gene
  GPIHBP1
• Long gene name
  glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1
• Synonyms gene name
  • GPI anchored high density lipoprotein binding protein 1
• Synonyms
  • LOC338328
  • GPI-HBP1
• Synonyms name
  • endothelial cell LPL transporter
• GenBank acession
  • AF088057
• Locus
  8q24.3
• Discovery year
  2008-02-07
• Entrez gene record
  338328
• Pubmed identfication
  • 12496272
  • 17883852
  • 17620854
  • 17403372
• RefSeq identity
  • NM_178172
• Classification
  • LY6/PLAUR domain containing
• VEGA ID
  OTTHUMG00000164950

Gene info

• Identity
  HGNC:12706
• Gene
  VNN2
• Long gene name
  vanin 2
• Synonyms
  • FOAP-4
  • GPI-80
• Synonyms name
  • pantetheinase
• GenBank acession
  • AB026705
• Locus
  6q23.2
• Discovery year
  1998-11-13
• Entrez gene record
  8875
• Pubmed identfication
  • 9790769
  • 11491533
• Classification
  • Vanin family
• VEGA ID
  OTTHUMG00000015588

Gene info

• Identity
  HGNC:4417
• Gene
  GNPDA1
• Long gene name
  glucosamine-6-phosphate deaminase 1
• Synonyms gene
  • GNPI
• Synonyms gene name
  • glucosamine-6-phosphate isomerase
• Synonyms
  • GNPDA
  • HLN
  • GPI
  • KIAA0060
• Synonyms name
  • glucosamine-6-phosphate deaminase
  • oscillin
• GenBank acession
  • AF048826
• Locus
  5q31.3
• Discovery year
  1999-09-09
• Entrez gene record
  10007
• Pubmed identfication
  • 9714720
  • 9438414
• RefSeq identity
  • NM_005471
• VEGA ID
  OTTHUMG00000129657

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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