SIRT1 (193-747 aa) (GST-tagged), Human recombinant
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Catalog number7264-25
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PricePlease ask
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Size25 units
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SynonymsNAD-dependent Deacetylase 1, SIR2L1, SIR2-like Protein 1, Sirtuin 1, Silent Information Regulator 2
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Alternative_namesNAD-dependent Deacetylase 1, SIR2L1, SIR2-like Protein 1, Sirtuin 1, Silent Information Regulator 2
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DescriptionRegulates the activity of the p53 tumor suppressor and inhibits apoptosis
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RecombinantYes
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SourceE. coli
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Purity by SDS PAGE≥60%
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AssaySDS-PAGE
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Biological activity≥ 550 pmol/min/mg
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Results≥ 550 pmol/min/mg
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Unit DefinitionOne unit is defined as the amount of enzyme required to produce 1 pmole of 7-amino-4-methylcoumarin/min at 25°C in 50 mM Tris-HCL, pH 8.0, 137 nM NaCl, 2.7 mM KCl, 1 mM MgCl2 containing 125 µM p53 amino acids 379-382 and 6 mM NAD+.
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Molecular Weight82.9 kDa
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Storage Temp-80°C
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ShippingDry Ice
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Shelf Life9 months
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AppearanceLiquid
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Physical form descriptionSupplied as a solution in 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol
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Background InformationThe sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. There are seven human sirtuins, which have been designated SIRT1-7. SIRT1, which is located in the nucleus, is the human sirtuin with the greatest homology to yeast Sir2 (Silent information regulator 2) and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. These results have significant implications regarding an important role of SIRT1 in modulating the sensitivity of cells in the p53-dependent apoptotic response and the possible effect in cancer therapy. Since the growth suppressive function of p53 is strongly enhanced by DNA damaging reagents, it is expected that inhibitors of SIRT1 may be effective anti-cancer drugs.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Additional sourceRecombinants or rec. proteins
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Grouprecombinants
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Gene target
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Gene symbolMGST3, SIRT1-AS, SIRT1, ANKRD1, MIR193A
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Short nameSIRT1 (193-747 aa) (GST-tagged), recombinant
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TechniqueRecombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
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SpeciesHuman, Humans
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Alternative namesirtuin 1 (193-747 aa) (GST-tagged), H. sapiens Rec.
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Alternative techniquerec
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Alternative to gene targetsirtuin 1, SIR2L1, SIRT1 and IDBG-75847 and ENSG00000096717 and 23411, keratin filament binding, nuclei, Sirt1 and IDBG-159441 and ENSMUSG00000020063 and 93759, SIRT1 and IDBG-629119 and ENSBTAG00000014023 and 613629
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Gene info
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Identity
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Gene
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Long gene namemicrosomal glutathione S-transferase 3
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1997-10-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Microsomal glutathione S-transferases
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameSIRT1 antisense RNA
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Locus
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Discovery year2015-09-23
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Entrez gene record
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Pubmed identfication
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Classification
- Antisense RNAs
Gene info
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Identity
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Gene
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Long gene namesirtuin 1
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Synonyms gene name
- sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1
- sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-03-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- eNoSC complex
- Sirtuins
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameankyrin repeat domain 1
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Synonyms gene name
- ankyrin repeat domain 1 (cardiac muscle)
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Synonyms
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GenBank acession
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Locus
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Discovery year2003-11-10
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Ankyrin repeat domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemicroRNA 193a
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Synonyms gene
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Synonyms gene name
- microRNA 193
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR193 family
MeSH Data
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Name
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ConceptScope note: The initial culturing of cells derived directly from fresh TISSUES.
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Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data