SIRT1 (193-747 aa) (GST-tagged), Human recombinant

• Catalog number
  7264-25
• Price
  Please ask
• Size
  25 units

• Synonyms
  NAD-dependent Deacetylase 1, SIR2L1, SIR2-like Protein 1, Sirtuin 1, Silent Information Regulator 2
• Alternative_names
  NAD-dependent Deacetylase 1, SIR2L1, SIR2-like Protein 1, Sirtuin 1, Silent Information Regulator 2
• Description
  Regulates the activity of the p53 tumor suppressor and inhibits apoptosis
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥60%
• Assay
  SDS-PAGE
• Biological activity
  ≥ 550 pmol/min/mg
• Results
  ≥ 550 pmol/min/mg
• Unit Definition
  One unit is defined as the amount of enzyme required to produce 1 pmole of 7-amino-4-methylcoumarin/min at 25°C in 50 mM Tris-HCL, pH 8.0, 137 nM NaCl, 2.7 mM KCl, 1 mM MgCl2 containing 125 µM p53 amino acids 379-382 and 6 mM NAD+.
• Molecular Weight
  82.9 kDa
• Storage Temp
  -80°C
• Shipping
  Dry Ice
• Shelf Life
  9 months
• Appearance
  Liquid
• Physical form description
  Supplied as a solution in 50 mM sodium phosphate, pH 7.2, containing 100 mM sodium chloride and 20% glycerol
• Background Information
  The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) and have been shown to catalyze a reaction that couples lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose from NAD+ and the abstracted acetyl group. There are seven human sirtuins, which have been designated SIRT1-7. SIRT1, which is located in the nucleus, is the human sirtuin with the greatest homology to yeast Sir2 (Silent information regulator 2) and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. These results have significant implications regarding an important role of SIRT1 in modulating the sensitivity of cells in the p53-dependent apoptotic response and the possible effect in cancer therapy. Since the growth suppressive function of p53 is strongly enhanced by DNA damaging reagents, it is expected that inhibitors of SIRT1 may be effective anti-cancer drugs.
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  SIRT1   193-747   GST-tagged  
• Gene symbol
  MGST3, SIRT1-AS, SIRT1, ANKRD1, MIR193A
• Short name
  SIRT1 (193-747 aa) (GST-tagged), recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  sirtuin 1 (193-747 aa) (GST-tagged), H. sapiens Rec.
• Alternative technique
  rec
• Alternative to gene target
  sirtuin 1, SIR2L1, SIRT1 and IDBG-75847 and ENSG00000096717 and 23411, keratin filament binding, nuclei, Sirt1 and IDBG-159441 and ENSMUSG00000020063 and 93759, SIRT1 and IDBG-629119 and ENSBTAG00000014023 and 613629

Gene info

• Identity
  HGNC:7064
• Gene
  MGST3
• Long gene name
  microsomal glutathione S-transferase 3
• Synonyms
  • GST-III
• Synonyms name
  • microsomal glutathione S-transferase III
  • microsomal GST-3
  • microsomal GST-III
• GenBank acession
  • AF026977
• Locus
  1q24.1
• Discovery year
  1997-10-16
• Entrez gene record
  4259
• Pubmed identfication
  • 9278457
• RefSeq identity
  • NM_004528
• Classification
  • Microsomal glutathione S-transferases
• VEGA ID
  OTTHUMG00000034627

Gene info

• Identity
  HGNC:51912
• Gene
  SIRT1-AS
• Long gene name
  SIRT1 antisense RNA
• Locus
  10q21.3
• Discovery year
  2015-09-23
• Entrez gene record
  106633813
• Pubmed identfication
  • 26324025
• Classification
  • Antisense RNAs

Gene info

• Identity
  HGNC:14929
• Gene
  SIRT1
• Long gene name
  sirtuin 1
• Synonyms gene name
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1
  • sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Synonyms
  • SIR2L1
• GenBank acession
  • AF083106
• Locus
  10q21.3
• Discovery year
  2001-03-20
• Entrez gene record
  23411
• Pubmed identfication
  • 10381378
• RefSeq identity
  • NM_012238
• Classification
  • eNoSC complex
  • Sirtuins
• VEGA ID
  OTTHUMG00000018340

Gene info

• Identity
  HGNC:15819
• Gene
  ANKRD1
• Long gene name
  ankyrin repeat domain 1
• Synonyms gene name
  • ankyrin repeat domain 1 (cardiac muscle)
• Synonyms
  • C-193
  • ALRP
  • CARP
  • CVARP
  • MCARP
• GenBank acession
  • X83703
• Locus
  10q23.31
• Discovery year
  2003-11-10
• Entrez gene record
  27063
• Pubmed identfication
  • 7730328
• RefSeq identity
  • NM_014391
• Classification
  • Ankyrin repeat domain containing
• VEGA ID
  OTTHUMG00000018734
• Locus Specific Databases
  • LRG_379

Gene info

• Identity
  HGNC:31563
• Gene
  MIR193A
• Long gene name
  microRNA 193a
• Synonyms gene
  • MIRN193
  • MIRN193A
• Synonyms gene name
  • microRNA 193
• Synonyms
  • hsa-mir-193
  • hsa-mir-193a
• Locus
  17q11.2
• Discovery year
  2004-04-23
• Entrez gene record
  406968
• RefSeq identity
  • NR_029710
• Classification
  • MicroRNA MIR193 family

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products