Sheep, L), HRP conjugated

• Catalog number
  AS10 1538
• Price
  Please ask
• Size
  1 mg

• Available ordering format
  Lyophilized
• Immunogen
  Purified rabbit IgG (H&L)
• Raised in
  Sheep
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Affinity purified sheep IgG
• How to reconstitute
  For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
• Storage condition
  Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
• Verified applications
  Contact Gentaur support center
• Connected products
  other HRP conjugated secondary antibodies against rabbit IgG (H&L)
• Recommended dilutions for use
  This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5 000 for Immunohistochemistry, 1:200-1:5000 for ELISA/Western blot.
• Molecular weight expected аpparent
  see datasheet
• Verified reactivity
  to be determined
• Possible reactivity
  to be determined
• No reactivity
  to be determined
• Supplementary information
  Contact Gentaur support center at [email protected]
• References
  Refer to PubMed
• Scientific context
  Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to HRP, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L).
• Notes
  Purity of this preparation is > 95% based on SDS-PAGE. Antibody concentration is 1.0 mg/ml. Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.1 % (v/v) Kathon CG as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.
• Protein number
  Refer to NCBI
• TAIR number
  Refer to NCBI
• Hyperlink
• Properties
  HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
• Conjugation
  HRP Conjugated

• Gene target
  conjugated  
• Short name
  , L), conjugated
• Technique
  antibody Conjugates
• Host
  Sheep
• Label
  HRP
• Species
  Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  ovine, L), horseradish peroxidase coupled

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