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Alternative name1
Anti-SHEEP ANTI HUMAN IgG3:FITC
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Alternative name2
Ovine ANTI HUMAN IgG3:FITC
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Alternative name3
Ovinae ANTI Homo sapiens IgG3:FITC
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Alternative name4
IgG3
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Alternative name5
N/A
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Other names
Ig gamma-3 chain C region; Ig gamma-3 chain C region; N/A; immunoglobulin heavy constant gamma 3 (G3m marker); HDC; Heavy chain disease protein
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Gene name
IgG3
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Gene name synonims
N/A
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Other gene names
IGHG3; IGHG3; IgG3; N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
Polyclonal IgG
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Clone
Polyclonal antibody
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Host organism
Sheep
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Species reactivity
Human
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Specificity and cross reactivity
IgG3 This item recognises human IgG3 and was shown to be specific by immunoelectrophoresis and double diffusion (Ouchterlony).
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Purification method
N/A
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Form Appearance
FITC (Ig Fraction - liquid)
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Concentration
Ig concentration 0.5 mg/ml
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Storage and shipping
+Store productone at +4 degrees Celsius., Store productone at -20 degrees Celsius. if preferred productone is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Shelf Life: 18 months from date of dispatch.
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Tested for
Immunofluorescence (IF)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Additional description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
