Sheep anti Bub1b-NT

• Catalog number
  X1162P
• Price
  Please ask
• Size
  250 μg

• Category
  Primary Antibodies
• Long description
  Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.
• Antibody come from
  Recombinant protein corresponding to amino acids 10-190 of the mouse Bub1b protein.
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide. Ammonium Sulfate Precipitation
• Clone
  Polyclonal
• Antigen antibody binding interaction
  Sheep anti Bub1b-NT Antibody
• Antibody is raised in
  Sheep
• Antibody s reacts with
  Mouse
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Application
  Western Blot, Immunohistochemistry
• Antibody s suited for
  Antibody detects Bub1b in mitotic cells seperated from NIH/3T3 cells by IP/Westen blot analysis (1-10 µg/ml). Detects a 130 kDa band. Optimal concentration should be evaluated by serial dilutions.
• Storage
  -20ºC
• Relevant references
  1. Myrie, K.A., et al. Mutation and expression analysis of human Bub1 and Bub1b in aneuploid breast cancer cell lines. Cancer Lett. 2000, 152, 193-199._x000B__x000B_2. Davenport, J.W., et al. The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner. Genomics 1999, 55, 113-117.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

• Gene target
  Bub1b-NT  
• Gene symbol
  NTF4
• Short name
  anti Bub1b-NT
• Technique
  anti, antibody to
• Host
  Sheep
• Isotype
  not specified
• Label
  unlabeled
• Species
  Sheep, The sheep (Ovis aries) ELISAs are detecting sheep proteins and antigens. Some epitopes will also recognize goat proteins. Sheep and goats are also used to produce polyclonals. An adult female sheep is referred to as a ewe a male as a ram, a castrated male as a wether, and a younger sheep as a lamb. Sheeps
• Alternative name
  ovine antibody to budding uninhibited by benzimidazoles 1 homolog b (yeast)-NT
• Alternative technique
  antibodies
• Alternative to gene target
  budding uninhibited by benzimidazoles 1 homolog beta (yeast), Bub1A and BUB1beta and BUBR1 and hBUBR1 and MAD3L and MVA1 and SSK1, BUB1B and IDBG-6103 and ENSG00000156970 and 701, transferase activity, nuclei, Bub1b and IDBG-198179 and ENSMUSG00000040084 and 12236, BT.25661 and IDBG-646208 and ENSBTAG00000007237 and 100851213,537027

Gene info

• Identity
  HGNC:8024
• Gene
  NTF4
• Long gene name
  neurotrophin 4
• Synonyms gene
  • NTF5
• Synonyms gene name
  • neurotrophin 5 (neurotrophin 4/5)
• Synonyms
  • NT-4/5
  • GLC1O
• Synonyms name
  • neurotrophic factor 4
• Locus
  19q13.33
• Discovery year
  1992-10-15
• Entrez gene record
  4909
• Pubmed identfication
  • 1496419
• RefSeq identity
  • NM_006179
• Classification
  • Neurotrophins
• VEGA ID
  OTTHUMG00000191728

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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