SHC (Tyr427) Antibody, Cy3 Conjugated

• Catalog number
  bs-3412R-Cy3
• Price
  Please ask
• Size
  0.1ml

• Long name
  SHC (Tyr427) Polyclonal Antibody, Cy3 Conjugated
• Category
  Conjugated Primary Antibodies
• Conjugation
  Cy3
• Host Organism
  Rabbit (Oryctolagus cuniculus)
• Target Antigen
  SHC Tyr427
• Specificity
  This is a highly specific antibody against SHC Tyr427.
• Modification
  Phosphorylation
• Modification site
  Tyr427
• Clonality
  Polyclonal
• Clone
  Polyclonal antibody
• Concentration
  1ug per 1ul
• Immunogen_range
  405-455/583
• Subcellular location
  Cytoplasm, Mitochondrion
• Source
  KLH conjugated synthetic phosphopeptide derived from human SHC around the phosphorylation site of Tyr427
• Gene ID number
  6464
• Swiss Prot
  P29353
• Tested applications
  IF(IHC-P)
• Recommended dilutions
  IF(IHC-P)(1:50-200)
• Crossreactivity
  Human, Mouse, Rat
• Crossreactive species details
  Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
• Antigen background
  Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in signaling downstream of the angiopoietin receptor TEK/TIE2, and plays a role in the regulation of endothelial cell migration and sprouting angiogenesis.
• Purification method
  This antibody was purified via Protein A.
• Storage conditions
  Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
• Excitation Emission
  512,550nm/570,615nm
• Synonyms
  SHC; SHCA; SHC-transforming protein 1; SHC-transforming protein 3; SHC-transforming protein A; Src homology 2 domain-containing-transforming protein C1; SH2 domain protein C1; SHC1
• Properties
  If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-SHC Tyr427 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
• Additional conjugation
  cy3 conjugation kit
• French translation
  anticorps

• Gene target
  SHC   Tyr427   Conjugated  
• Gene symbol
  SHC1, SHC3
• Short name
  Anti-SHC Tyr427
• Technique
  Antibody, antibodies against human proteins, antibodies for, antibody Conjugates
• Isotype
  Immunoglobulin G (IgG)
• Label
  Cy3
• Alternative name
  Anti-SHC Tyr427 PAb Cy3
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10840
• Gene
  SHC1
• Long gene name
  SHC adaptor protein 1
• Synonyms gene
  • SHC
• Synonyms gene name
  • SHC (Src homology 2 domain-containing) transforming protein 1
• Synonyms
  • p66
  • ShcA
• GenBank acession
  • U73377
• Locus
  1q21.3
• Discovery year
  1993-02-17
• Entrez gene record
  6464
• Pubmed identfication
  • 1623525
• RefSeq identity
  • NM_183001
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000037295

Gene info

• Identity
  HGNC:18181
• Gene
  SHC3
• Long gene name
  SHC adaptor protein 3
• Synonyms gene name
  • src homology 2 domain containing transforming protein C3
  • SHC (Src homology 2 domain containing) transforming protein 3
• Synonyms
  • N-Shc
  • NSHC
  • SHCC
• GenBank acession
  • D84361
• Locus
  9q22.1
• Discovery year
  2003-11-12
• Entrez gene record
  53358
• Pubmed identfication
  • 8808684
• RefSeq identity
  • NM_016848
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000020179

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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