SHC Antibody

• Catalog number
  R30997
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  SHC
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, IHC-F, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the SHC antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This SHC antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P29353
• Purity
  Antigen affinity
• Description
  SHC-transforming protein 1, also known as SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. By Southern analysis of somatic cell hybrids followed by both isotopic and fluorescence in situ hybridization, Huebner et al.(1994) assigned the SHC1 gene to 1q21. Yulug et al.(1995) used fluorescence in situ hybridization to map the SHC1 gene to 1q21. By the same method, an SHC-related sequence (SHCL1) was mapped to 17q21-q22. By FISH analysis and direct sequencing of vectorette library PCR products, Harun et al.(1997) identified SHC1P1, a 3.2-kb processed pseudogene, in Xq12-q13.1. SHC1P1 is 85% identical to mouse SHC p66. Reporter assays showed FKHRL1 transactivates CAT, suggesting a capacity to augment antioxidant scavenging. Nemoto and Finkel(2002) concluded that there is an important functional relationship between forkhead proteins(e.g., FKHRL1), SHC1, and intracellular oxidants, all of which are though to be involved in the aging process in worms and mammals.
• Immunogen
  An amino acid sequence from the C-terminus of human SHC (DPSYVNVQNLDKARQAV) was used as the immunogen for this SHC antibody.
• Storage
  After reconstitution, the SHC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  SHC  
• Gene symbol
  SHC1, SHC3
• Short name
  Anti-SHC
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to SHC
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:10840
• Gene
  SHC1
• Long gene name
  SHC adaptor protein 1
• Synonyms gene
  • SHC
• Synonyms gene name
  • SHC (Src homology 2 domain-containing) transforming protein 1
• Synonyms
  • p66
  • ShcA
• GenBank acession
  • U73377
• Locus
  1q21.3
• Discovery year
  1993-02-17
• Entrez gene record
  6464
• Pubmed identfication
  • 1623525
• RefSeq identity
  • NM_183001
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000037295

Gene info

• Identity
  HGNC:18181
• Gene
  SHC3
• Long gene name
  SHC adaptor protein 3
• Synonyms gene name
  • src homology 2 domain containing transforming protein C3
  • SHC (Src homology 2 domain containing) transforming protein 3
• Synonyms
  • N-Shc
  • NSHC
  • SHCC
• GenBank acession
  • D84361
• Locus
  9q22.1
• Discovery year
  2003-11-12
• Entrez gene record
  53358
• Pubmed identfication
  • 8808684
• RefSeq identity
  • NM_016848
• Classification
  • SH2 domain containing
• VEGA ID
  OTTHUMG00000020179

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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