Sec21p, gamma subunit, COP vesicles

• Catalog number
  AS08 327
• Price
  Please ask
• Size
  200 µl

• Available ordering format
  Lyophilized
• Immunogen
  GST fusion of a part of recombinant Sec21 of Arabidopsis thaliana Q0WW26, At4g34450
• Raised in
  Rabbit
• Clonality
  Polyclonal
• Clone
  Polyclonal
• Purification
  Serum
• How to reconstitute
  For reconstitution add 200 µl of sterile water.
• Storage condition
  store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
• Verified applications
  western blot (WB), immunofluorescence (IF)
• Connected products
  AS10 683 | anti-HDEL | endoplasmic reticulum retention signa, 100 µgAS10 683-25 | anti-HDEL | endoplasmic reticulum retention signal, 25 µgAS08 325 | anti-Arf1 | ADP-ribosylation factor 1, Golgi marker, rabbit antibodyPlant protein extraction bufferSecondary antibodies
• Recommended dilutions for use
  1: 1000 with standard ECL (WB), 1: 1000 (IF)
• Molecular weight expected аpparent
  98 kDa
• Verified reactivity
  Arabidopsis thaliana, Zea mays
• Possible reactivity
  Brachypodium distachyon, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Citrus clementina, Coffea canephora, Eutrema salsugineum, Glycine max, Glycine soja, Hordeum vulgare var. distichum, Medicago truncatula, Oryza sativa, Populus trichocarpa, Prunus persica, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Vitis vinifera, Zea mays,
• No reactivity
  Nicotiana tabacum, Microsporidia sp.
• Supplementary information
  This antibody can be used as a Golgi marker in immunolocalization and as a marker of COP1 in Western blot.References describing immunolocalization (IF) and (IG) studies:Pimpl et al (2000). In Situ Localization and in Vitro Induction of Plant COPI-Coated Vesicles. Plant Cell. 2000 Nov;12(11):2219-36. Ritzenthaler et al. (2002). Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera. Plant Cell. 2002 Jan;14(1):237-61.
• References
  Wattelet-Boyer et al. (2016). Enrichment of hydroxylated C24- and C26-acyl- chain sphingolipids mediates PIN2 apical sorting at trans-Golgi network subdomains. Nat Commun. 2016 Sep 29;7:12788. doi: 10.1038/ncomms12788. Derbyshire et al. (2015). Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis. Plant Cell. 2015 Oct 2. pii: tpc.15.00314.Tanaka et al. (2013). Cell Polarity and Patterning by PIN Trafficking through Early Endosomal Compartments in Arabidopsis thaliana. PLoS Genet. May;9(5). (immunolocalization). Hopff et al. (2013). The plasma membrane proteome of maize roots grown under low and high iron conditions. J Proteomics Jan 24. Pimpl et al (2000). In situ localization and in vitro induction of plant COPI-coated vesicles. Plant Cell. 2000 Nov;12(11):2219-36.
• Scientific context
  Sec21p is a constituent of the COPI vesicle coatomer.
• Notes
  None
• Protein number
  Q0WW26
• TAIR number
  AT4G34450
• Hyperlink

• Gene target
  Sec21p   gamma   subunit   COP   vesicles  
• Gene symbol
  COPB2, COPG2, COPA, COPE, CARD16
• Short name
  Sec21p, gamma subunit, COP vesicles
• Alternative name
  Sec21p, g functionnal sequence, COP vesicles

Gene info

• Identity
  HGNC:2232
• Gene
  COPB2
• Long gene name
  COPI coat complex subunit beta 2
• Synonyms gene name
  • coatomer protein complex, subunit beta 2 (beta prime)
  • coatomer protein complex subunit beta 2 (beta prime)
• Synonyms
  • beta'-COP
  • betaprime-COP
• Synonyms name
  • coatomer protein complex subunit beta prime
• GenBank acession
  • BC000326
• Locus
  3q23
• Discovery year
  1999-04-23
• Entrez gene record
  9276
• Pubmed identfication
  • 9858824
• RefSeq identity
  • NM_004766
• Classification
  • WD repeat domain containing
  • COPI coat complex
• VEGA ID
  OTTHUMG00000159959

Gene info

• Identity
  HGNC:2237
• Gene
  COPG2
• Long gene name
  COPI coat complex subunit gamma 2
• Synonyms gene name
  • coatomer protein complex, subunit gamma 2
• Synonyms
  • 2-COP
• Synonyms name
  • coat protein, nonclathrin, gamma-2-cop
• GenBank acession
  • AF157833
• Locus
  7q32.2
• Discovery year
  1999-12-17
• Entrez gene record
  26958
• Pubmed identfication
  • 10556286
  • 10995575
• RefSeq identity
  • NM_012133
• Classification
  • Clathrin/coatomer adaptor, adaptin-like, N-terminal domain containing
  • COPI coat complex
• VEGA ID
  OTTHUMG00000155364

Gene info

• Identity
  HGNC:2230
• Gene
  COPA
• Long gene name
  COPI coat complex subunit alpha
• Synonyms gene name
  • coatomer protein complex, subunit alpha
• Synonyms
  • HEP-COP
• Synonyms name
  • proxenin
  • xenin
• GenBank acession
  • U24105
• Locus
  1q23.2
• Discovery year
  1996-11-15
• Entrez gene record
  1314
• Pubmed identfication
  • 8647451
• RefSeq identity
  • NM_004371
• Classification
  • WD repeat domain containing
  • COPI coat complex
  • Receptor ligands
• VEGA ID
  OTTHUMG00000033111
• Locus Specific Databases
  • LRG_1336

Gene info

• Identity
  HGNC:2234
• Gene
  COPE
• Long gene name
  COPI coat complex subunit epsilon
• Synonyms gene name
  • coatomer protein complex, subunit epsilon
• Synonyms
  • epsilon-COP
• GenBank acession
  • AJ131182
• Locus
  19p13.11
• Discovery year
  1999-09-28
• Entrez gene record
  11316
• Pubmed identfication
  • 10469566
• RefSeq identity
  • NM_007263
• Classification
  • COPI coat complex
• VEGA ID
  OTTHUMG00000183052

Gene info

• Identity
  HGNC:33701
• Gene
  CARD16
• Long gene name
  caspase recruitment domain family member 16
• Synonyms gene name
  • caspase recruitment domain family, member 16
• Synonyms
  • COP1
  • COP
  • PSEUDO-ICE
  • LLID-114769
• Locus
  11q22.3
• Discovery year
  2008-09-15
• Entrez gene record
  114769
• Pubmed identfication
  • 11432859
  • 11536016
• Classification
  • Caspase recruitment domain containing
• VEGA ID
  OTTHUMG00000166157

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