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Available ordering format
Lyophilized
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Immunogen
purified goat IgG (H&L), whole molecule
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Raised in
Rabbit
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Clonality
Polyclonal
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Clone
Polyclonal
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Purification
Affinity purified Rabbit IgG
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How to reconstitute
For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
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Storage condition
Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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Verified applications
Contact Gentaur support center
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Connected products
other dylight anti-goat secondary antibodies
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Recommended dilutions for use
1:20 - 1:2,000 for most applications
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Molecular weight expected аpparent
see datasheet
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Verified reactivity
to be determined
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Possible reactivity
to be determined
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No reactivity
to be determined
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Supplementary information
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References
to be added when available
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Scientific context
Rabbit anti-goat IgG (H&L) - DyLight®650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to Goat IgG (H&L) in immunological assays.DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase goat IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.
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Notes
Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on goat IgG light chains on all goat immunoglobulinsNo reactivity is observed to: non-immunoglobulin goat serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
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Protein number
Refer to NCBI
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TAIR number
Refer to NCBI
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About
Rabbits are used for polyclonal antibody production by agisera. Rabbit antibodies are very stable and can be stored for several days at room temperature. agisera adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
