S-adenosylmethionine synthetase (AdoMetS), Active, E. coli Recombinant

  • Catalog number
    P1211-50
  • Price
    Please ask
  • Size
    50 ug
  • Synonyms
    Methionine adenosyltransferase, AdoMet synthetase, MAT
  • Alternative_names
    Methionine adenosyltransferase, AdoMet synthetase, MAT
  • Description
    Catalyzes the formation of AdoMet from methionine and ATP
  • Recombinant
    Yes
  • Source
    E.coli
  • Purity by SDS PAGE
    > 90%
  • Assay
    SDS-PAGE
  • Activity Specifications test method
    Measured by its ability to converts 1 umole of L-methionine and ATP to S-adenosyl-L-methionine (AdoMet), inorganic phosphate (Pi), and pyrophosphate (PPi) at 37ºC and pH 7
  • Biological activity
    Specific activity ≥50 mU/mg.
  • Results
    ≥10 U/mg
  • Unit Definition
    One unit of Adenosylmethionine synthetase enzyme converts 1 umole of L-methionine and ATP to S-adenosyl-L-methionine (AdoMet), inorganic phosphate (Pi), and pyrophosphate (PPi) at 37ºC and pH 7.
  • Molecular Weight
    43 kDa
  • Storage Temp
    -20°C
  • Shipping
    Gel Pack
  • Shelf Life
    12 months
  • Concentration
    3.6 mg/ml
  • Appearance
    Liquid
  • Physical form description
    Proprietary formulation
  • Background Information
    S-Adenosylmethionine synthase, (AdoMetS), (E.C. 2.5.1.6) catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. BioVision’s active AdoMetS is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Additional description
    E. coli recombinant proteins are affinity purified and supplied lyophilized. For human research only.
  • Additional source
    Recombinants or rec. proteins
  • Group
    recombinants
  • Gene target
  • Short name
    S-adenosylmethionine synthetase (AdoMetS), Active, Recombinant
  • Technique
    coli recombinant, Recombinant, from e. Coli, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
  • Host
    e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
  • Species
    E. coli, E. coli
  • Alternative name
    S-adenosylmethionine synthetase (AdoMetS), functionnal, E. coli Rec.
  • Alternative technique
    rec, escherichia
MeSH Data
  • Name
  • Concept
    Scope note: The initial culturing of cells derived directly from fresh TISSUES.
  • Tree numbers
    • E01.370.225.500.223.500
    • E05.200.500.265.500
    • E05.242.223.500
    • E05.481.500.249.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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