S-adenosylmethionine synthetase (AdoMetS), Active, E. coli Recombinant
-
Catalog number
P1211-50
-
Price
Please ask
-
Size
50 ug
-
-
Synonyms
Methionine adenosyltransferase, AdoMet synthetase, MAT
-
Alternative_names
Methionine adenosyltransferase, AdoMet synthetase, MAT
-
Description
Catalyzes the formation of AdoMet from methionine and ATP
-
Recombinant
Yes
-
Source
E.coli
-
Purity by SDS PAGE
> 90%
-
Assay
SDS-PAGE
-
Activity Specifications test method
Measured by its ability to converts 1 umole of L-methionine and ATP to S-adenosyl-L-methionine (AdoMet), inorganic phosphate (Pi), and pyrophosphate (PPi) at 37ºC and pH 7
-
Biological activity
Specific activity ≥50 mU/mg.
-
Results
≥10 U/mg
-
Unit Definition
One unit of Adenosylmethionine synthetase enzyme converts 1 umole of L-methionine and ATP to S-adenosyl-L-methionine (AdoMet), inorganic phosphate (Pi), and pyrophosphate (PPi) at 37ºC and pH 7.
-
Molecular Weight
43 kDa
-
Storage Temp
-20°C
-
Shipping
Gel Pack
-
Shelf Life
12 months
-
Concentration
3.6 mg/ml
-
Appearance
Liquid
-
Physical form description
Proprietary formulation
-
Background Information
S-Adenosylmethionine synthase, (AdoMetS), (E.C. 2.5.1.6) catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme. BioVision’s active AdoMetS is suitable for functional assays, high-throughput screening and preclinical studies in drug discovery.
-
Handling
Centrifuge the vial prior to opening.
-
Usage
For Research Use Only! Not to be used in humans
-
-
Additional description
E. coli recombinant proteins are affinity purified and supplied lyophilized. For human research only.
-
Additional source
Recombinants or rec. proteins
-
Group
recombinants
-
Gene target
-
Short name
S-adenosylmethionine synthetase (AdoMetS), Active, Recombinant
-
Technique
coli recombinant, Recombinant, from e. Coli, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
-
Host
e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
-
Species
E. coli, E. coli
-
Alternative name
S-adenosylmethionine synthetase (AdoMetS), functionnal, E. coli Rec.
-
Alternative technique
rec, escherichia
-
MeSH Data
-
Name
-
Concept
Scope note:
The initial culturing of cells derived directly from fresh TISSUES.
-
Tree numbers
- E01.370.225.500.223.500
- E05.200.500.265.500
- E05.242.223.500
- E05.481.500.249.500
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products